| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The DNA Replication machinery, or replisome is displaced from chromatin at the end of DNA replication and during the collapse of stalled replication forks. In this study, I aimed to elucidate the molecular mechanism of the replisome displacement during fork collapse by utilizing cell-free DNA replication system, and found that the replisome displacement was efficiently induced at stalled forks by enforcing cell cycle transit from interphase into mitotic phase. I also demonstrated that this mitotic displacement pathway is a novel one that requires CDK activity, ubiquitylation and Mre11 nuclease activatey.
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