| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The X-ray structure of archaeal aTrm11, which is homologous to the catalytic unit of Trm11 in eukaryotic tRNA (m2G10) methyltransferase Trm11-Trm112, is determined, and the structure-guided biochemical analysis provides mechanistic insight into site specificity of archaeal Trm11 and furher proposes a molecular ruler mechanism by which the Trm11 defines the distance and angle from the end of 3'-CCA in substrate tRNA to methylation site. The structural and functional analyses of RNA-splicing endonuclease (EndA) from hyperthermophilic archaeon Methanopyrus kandleri have demonstrated that the MKA EndA consists of heterodimer （αβ)2 and shows a constrain substrate specificity.
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