| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Cell division at M-phase is controlled by several mitotic kinases. Greatwall (Gwl) is an essential mitotic kinase. Gwl down regulates PP2A-B55 phosphatase through Arpp19/Ensa phosphorylation at the mitotic entry. Gwl also controls the mitotic progression but its substrate(s) is/are unknown. This research aimed identification of the substrate(s). 15 candidates are found by chemical genetics method in our previous work. One of the candidates, that would have affinity to chromosomes, is focused to analyze. Questions are whether it is the direct target of Gwl and what is the role in the mitotic progression. A fragment peptide of the candidate was phosphorylated by active Gwl kinase in vitro. Antibody against the fragment detected a band at predicted size of the candidate. The candidate was phosophorylated at M-phase depending not only on Gwl but also on Cdk1. Several results suggested that the candidate might control chromosome segregation.
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