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In vivo imaging of histone modification dynamics during ZGA

Research Project

Project/Area Number 15K07157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genetics/Chromosome dynamics
Research InstitutionTokyo Institute of Technology

Principal Investigator

Sato Yuko  東京工業大学, 科学技術創成研究院, 助教 (70435882)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsエピジェネティクス / 胚性ゲノム活性化 / ライブイメージング / 転写 / ヒストン修飾 / ゼブラフィッシュ
Outline of Final Research Achievements

To visualize the onset of ZGA in living embryos, we injected Fab specific to Ser2 phosphorylated RNA polymerase II (RNAP2 Ser2ph) into fertilized eggs. Fluorescence microscopy showed that RNAP2 Ser2ph-Fab became concentrated in nuclei at around 1024-cell stages, indicating that global gene activation was faithfully detected by our system. We also detected a few RNAP2 Ser2ph foci corresponded to the clusters of miR-430 genes, which are highly expressed in early stages.
To detect changes in the levels of histone modifications during ZGA, modification-specific Fabs were injected with RNAP2 Ser2ph-Fab. We found that H3K27ac was the most dynamically increased along with ZGA. The nuclear accumulation of H3K27ac-Fab started earlier than that of RNAP2 Ser2ph-Fab. In addition, H3K27ac also appeared in miR-430 foci earlier than RNAP2 Ser2ph. Inhibition of bromodomain-containing proteins diminished RNAP2Ser2ph accumulation. These results suggest that H3K27ac promotes ZGA and drives transcription.

Academic Significance and Societal Importance of the Research Achievements

これまで生きた胚の中で発生過程におけるヒストン修飾レベルを調べる方法はなかった。今回初めて転写活性化とヒストン修飾動態を同時に観察することに成功した。本研究で開発した方法は、今後個体レベルでのクロマチン動態研究に貢献することが期待できる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Open Access: 4 results,  Peer Reviewed: 2 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Book (1 results)

  • [Int'l Joint Research] MPI-CBG(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] HHMI, Janelia Research Campus(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] MPI-CBG(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] MPI-CBG(ドイツ)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] MPI-CBG(ドイツ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Quantitative measurements of chromatin modification dynamics during zygotic genome activation2019

    • Author(s)
      Yuko Sato, Lennart Hilbert, Haruka Oda, Yinan Wan, John M. Heddleston, Teng-Leong Chew, Vasily Zaburdaev, Philipp Keller, Timothee Lionnet, Nadine Vastenhouw, Hiroshi Kimura
    • Journal Title

      bioRxiv

      Volume: 601393 Pages: 601393-601393

    • DOI

      10.1101/601393

    • Related Report
      2018 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Transcription organizes euchromatin similar to an active microemulsion2018

    • Author(s)
      Hilbert Lennart、Sato Yuko、Kimura Hiroshi、J?licher Frank、Honigmann Alf、Zaburdaev Vasily、Vastenhouw Nadine
    • Journal Title

      bioRxiv

      Volume: - Pages: 234112-234112

    • DOI

      10.1101/234112

    • Related Report
      2018 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes2018

    • Author(s)
      Sato Yuko、Stasevich Timothy J.、Kimura Hiroshi
    • Journal Title

      Methods in Molecular Biology

      Volume: 1861 Pages: 91-102

    • DOI

      10.1007/978-1-4939-8766-5_8

    • ISBN
      9781493987658, 9781493987665
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A genetically encoded probe for live-cell imaging of H4K20 monomethylation2016

    • Author(s)
      Sato Y., Kujirai T., Arai R., Asakawa H., Ohtsuki C., Horikoshi N., Yamagata K., Ueda J., Nagase T., Haraguchi T., Hiraoka Y., Kimura A., Kurumizaka H., Kimura, H.
    • Journal Title

      J. Mol. Biol.

      Volume: 428 Issue: 20 Pages: 3885-3902

    • DOI

      10.1016/j.jmb.2016.08.010

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histone Acetylation on <i>Drosophila</i> Polytene Chromosomes Visualized by Mintbody2015

    • Author(s)
      Sato Y, Mukai M, Kimura H
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 4 Pages: 383-384

    • DOI

      10.1508/cytologia.80.383

    • NAID

      130005118546

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2015 Research-status Report
    • Open Access
  • [Presentation] 細胞内抗体Mintbodyを用いた生細胞エピジェネティクス解析2018

    • Author(s)
      佐藤優子、木村 宏
    • Organizer
      第12回 日本エピジェネティクス研究会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Zygotic genome activation is dynamically mediated by histone acetylation in zebrafish2018

    • Author(s)
      Yuko Sato, Lennart Hilbert, Vasily Zaburdaev, Nadine Vastenhouw, Hiroshi Kimura
    • Organizer
      Cold Spring Harbor Laboratory “Nuclear Organization & Function”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Zygotic genome activation is dynamically mediated by histone acetylation2017

    • Author(s)
      Yuko Sato, Lennart Hilbert, Vasily Zaburdaev, Nadine Vastenhouw, and Hiroshi Kimura
    • Organizer
      EMBO workshop "Awakening of the genome: The maternal-to-zygotic transition"
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Zygotic genome activation is dynamically mediated by histone acetylation2017

    • Author(s)
      Yuko Sato, Lennart Hilbert, Vasily Zaburdaev, Nadine Vastenhouw, and Hiroshi Kimura
    • Organizer
      第11回日本エピジェネティクス研究会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ゼブラフィッシュ胚ゲノム活性化におけるヒストン修飾動態の意義2017

    • Author(s)
      Yuko Sato, Lennart Hilbert, Vasily Zaburdaev, Nadine Vastenhouw, and Hiroshi Kimura
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Visualizing the inactive X chromosome in vivo by using H4K20me1-specific intracellular antibody probe2015

    • Author(s)
      Sato Y, Kimura H
    • Organizer
      EMBL Conference “Nuclear structure and dynamics”
    • Place of Presentation
      Isle sur Sorgue (フランス)
    • Year and Date
      2015-10-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Visualizing The Inactive X Chromosome in Living Cells by Using H4K20me1-mintbody2015

    • Author(s)
      Sato Y, Kimura H
    • Organizer
      International Symposium on Chromatin Structure, Dynamics, and Function
    • Place of Presentation
      淡路夢舞台国際会議場
    • Year and Date
      2015-08-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゼブラフィッシュ胚性ゲノム活性化に伴うヒストン修飾動態のin vivoイメージング2015

    • Author(s)
      佐藤優子、木村宏
    • Organizer
      第9回 日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター 一橋講堂
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
  • [Book] DNA Replication, Recombination, and Repair (Fumio Hanaoka and Kaouru Sugawara ed)2016

    • Author(s)
      Kimura H, Sato Y
    • Total Pages
      555
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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