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Phylogenetic inference using genome-scale sequence data

Research Project

Project/Area Number 15K07187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biodiversity/Systematics
Research InstitutionKagawa University

Principal Investigator

Takezaki Naoko  香川大学, 総合生命科学研究センター, 教授 (30398036)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords分子系統樹 / 分子時計 / 多数遺伝子 / 四足動物 / 分岐時間推定 / 多遺伝子データ / 分岐年代推定 / 進化速度 / 分子系統 / シーラカンス / アウトグループ / 肺魚 / phylogenomics / coelacanth / lungfish / tetrapod
Outline of Final Research Achievements

Using genome-scale gene data sets, the phylogenetic relationships of coelacanths, lungfishes, and tetrapods were investigated. The results showed that incorrect tree topologies were generated by using ray-finned fish with high divergence level as outgroup, even though the ray-finned fish is taxonomically more closely related to the three groups than the cartilaginous fish. By using slowly evolving ray-finned fish the sister relationship of lungfishes and tetrapods was strongly supported.

Using the genome-scale data, variation of amino acid substitution rate in different time periods was investigated. The result suggested that the rate in the ancestral lineage was higher than those in peripheral lineages.

Academic Significance and Societal Importance of the Research Achievements

シーラカンス、肺魚、四足動物の系統関係を決定することは四足動物の進化を考える上で重要である。本研究では肺魚がシーラカンスよりも四足動物に近縁であることを確立した。塩基やアミノ酸の置換速度は、生物の寿命、代謝、環境など様々な要因に影響を受ける。また、分子時計を用いた分岐時間推定でも置換速度の変化についての仮定が推定値に影響することが知られている。置換速度の長期的変化を調べた研究は少ないが、本研究では硬骨脊椎動物では祖先系統でアミノ酸置換速度が高く、末端の系統で置換速度が低下する傾向があることを示した。これは、生物のlife historyの変化や分子分岐時間推定方法に示唆を与えるものである。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 4 results)

  • [Int'l Joint Research] Temple University(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Global rate variation in bony vertebrates2018

    • Author(s)
      Naoko Takezaki
    • Journal Title

      Genome Biol Evol

      Volume: 10 Issue: 7 Pages: 1803-1815

    • DOI

      10.1093/gbe/evy125

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Support for Lungfish as the Closest Relative of Tetrapods by Using Slowly Evolving Ray-Finned Fish as the Outgroup2017

    • Author(s)
      Naoko Takezaki, Hidenori Nishihara,
    • Journal Title

      Genome Biology and Evolution

      Volume: 9 Pages: 93-101

    • DOI

      10.1093/gbe/evw288

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Global Rate Variation in Bony Vertebrates.2018

    • Author(s)
      Naoko Takezaki
    • Organizer
      SMBE
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Global Rate Variation in Bony Vertebrates.2018

    • Author(s)
      竹崎 直子
    • Organizer
      日本進化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Resolving the phylogenetic position of coelacanth2017

    • Author(s)
      Naoko Takezaki and Hidenori Nishihara
    • Organizer
      International Symposium on Molecular Evolution and Medicine
    • Related Report
      2017 Research-status Report
  • [Presentation] シーラカンスの系統学的位置2017

    • Author(s)
      竹崎直子
    • Organizer
      日本進化学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Support for Lungfish as the Closest Relative of Tetrapods by Using Slowly Evolving Ray-Finned Fish as the Outgroup.2017

    • Author(s)
      Naoko Takezaki and Hidenori Nishihara
    • Organizer
      Society of Molecular Biology and Evolution
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Support for Lungfish as the Closest Relative of Tetrapods by Using Slowly Evolving Ray-Finned Fish as the Outgroup2016

    • Author(s)
      Naoko Takezaki, Hidenori Nishihara
    • Organizer
      日本遺伝学会
    • Place of Presentation
      三島
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Resolving the Phylogenetic Position of Coelacanth: The Closest Relative Is Not Always the Most Appropriate Outgroup2016

    • Author(s)
      Naoko Takezaki
    • Organizer
      SMBE 2016
    • Place of Presentation
      Gold Coast, Australia
    • Year and Date
      2016-07-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] シーラカンス、肺魚、四足動物の系統関係2015

    • Author(s)
      Naoko Takezaki, Hidenori Nishihara
    • Organizer
      日本遺伝学会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Phylogenetic relationship of coelacanths, lungfishes, and tetrapods2015

    • Author(s)
      Naoko Takezaki, Hidenori Nishihara
    • Organizer
      Society of Molecular Biology and Evolution
    • Place of Presentation
      ウィーン
    • Year and Date
      2015-07-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2022-01-24  

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