Investigation of genomic region involved in pathogenicity of Verticillium dahliae on tomato.

• Project/Area Number: 15K07309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Chiba University
• Principal Investigator: USAMI Toshiyuki 千葉大学, 大学院園芸学研究科, 准教授 (50334173)
• Research Collaborator: IIDA Naoko ; NAKAO Keisuke ; SUZUKI Nana
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 植物病害 / 植物病原性菌類 / 病原性 / 糸状菌 / 真菌 / 病原菌 / 子のう菌 / 宿主範囲 / 植物病理学 / 植物病原菌類 / 植物保護 / 植物防疫

Outline of Final Research Achievements

Pathogenicity mechanisms of Verticillium dahliae, a causal agent of wilt disease of various dicot plants, are unknown. In this study, genetic recombination between two strains of V. dahliae was performed to find a genomic region involved in pathogenicity of this fungus on tomato. And it was found that a genomic region (approx. 113 kbp) completely accorded with pathogenicity of V. dahliae on tomato. Therefore, this genomic region possibly includes some gene(s) involved in pathogenicity of V. dahliae on tomato.

Research Products

• [Journal Article] Race 2 of Verticillium dahliae infecting tomato in Japan can be split into two races with differential pathogenicity on resistant rootstocks. (2017)
  • Author(s): Usami T, Momma N, Kikuchi S, Watanabe H, Hayashi A, Mizukawa M, Yoshino K, Ohmori Y.
  • Journal Title: Plant Pathology Volume: 66 Issue: 2 Pages: 230-238
  • DOI: 10.1111/ppa.12576
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Verticillium dahliae のピーマンに対する病原性を決定する染色体の推定 (2018)
  • Author(s): 今野義規・狩野晃一・菊池琴乃・宇佐見俊行
  • Organizer: 平成30年度 日本植物病理学会大会
• [Presentation] トマト半身萎凋病菌の病原性決定染色体の推定と解析３ (2017)
  • Author(s): 濱野あやめ・中尾圭佑・鈴木那奈・宇佐見俊行
  • Organizer: 平成29年度日本植物病理学会大会
  • Place of Presentation: アイーナいわて県民情報交流センター（盛岡市）
  • Year and Date: 2017-04-26
• [Presentation] トマト半身萎凋病菌の病原性決定染色体の推定と解析1 (2016)
  • Author(s): 中尾圭佑・飯田尚子・宇佐見俊行
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県，岡山市）
  • Year and Date: 2016-03-21
• [Presentation] トマト半身萎凋病菌の病原性決定染色体の推定と解析2 (2016)
  • Author(s): 鈴木那奈・大村祐輔・宇佐見俊行
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県，岡山市）
  • Year and Date: 2016-03-21