Development of stealth-nanoparticles avoiding animal immune system that mimics the cell surface of filamentous fungi.
| Project/Area Number |
15K07348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
Yoshimi Akira 東北大学, 未来科学技術共同研究センター, 准教授 (60436102)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE Keietsu 東北大学, 農学研究科, 教授 (50312624)
KAWAKAMI Kazuyoshi 東北大学, 医学系研究科, 教授 (10253973)
TAKAMI Seiichi 東北大学, 多元物質科学研究所, 准教授 (40311550)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糸状菌 / 細胞壁 / ステルス素材 / α-1,3-グルカン / オリゴ糖合成 / 免疫細胞応答試験 / マウス免疫試験 / ステルス新素材 / オリゴ糖酵素合成 / 免疫応答評価 / オリゴ糖 / 転移酵素合成 / マウス試験 / 医療用ナノ粒子 / 亜臨界水分解 |
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| Outline of Final Research Achievements |
This study aims to create medical nanoparticles with stealth function using α - 1,3 - glucan (AG) and hydrophobin (HP) which are novel stealth materials derived from filamentous fungi. The results obtained in this study are as follows.
1) Determination of purification conditions for AG and AG oligosaccharides. 2) Confirmation of the stealth function of AG and AG oligosaccharides. 3) Confirmation of non-interaction between AG and HP. 4) Determination of enzymatic synthesis conditions of AG and AG oligosaccharides. 5) Demonstration of the stealth function of AG in vivo. 6) Evaluation of adsorption conditions of AG oligosaccharides and the particles.7) Discovery of galactosaminogalactan (GAG) as hyphal adhesion factor in Aspergillus oryzae. 8) Demonstration of additive interactions on the stealth function of AG and GAG.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexB gene encoding a subtilisin-like processing protease in Aspergillus oryzae2016
Author(s)
Osamu Mizutani, Matsuko Shiina, Akira Yoshimi, Motoaki Sano, Takeshi Watanabe, Youhei Yamagata, Tasuku Nakajima, Katsuya Gomi, and Keietsu Abe
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: published online
Issue: 9
Pages: 1-11
DOI
Related Report
Peer Reviewed
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[Presentation] Comparative analysis of the function of α-1,3-glucan syntheses, AgsA and AgsB, in Aspergillus nidulans.2017
Author(s)
Ken Miyazawa, Akira Yoshimi, Shigekazu Yano, Shin Kasahara, Humihiko Hasegawa, Keietsu Abe
Organizer
29th Fungal Genetic Conference
Place of Presentation
Pacific Grove, California, USA
Year and Date
2017-03-14
Related Report
Int'l Joint Research / Invited
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[Presentation] Comparative analysis of the function of α-1,3-glucan syntheses, AgsA and AgsB, in Aspergillus nidulans.2017
Author(s)
Ken Miyazawa, Akira Yoshimi, Shigekazu Yano, Shin Kasahara, Humihiko Hasegawa, Keietsu Abe
Organizer
14th International Aspergillus meeting
Place of Presentation
Pacific Grove, California, USA
Year and Date
2017-03-13
Related Report
Int'l Joint Research
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