Analysis and application of prophage-mediated gene reconstitution
| Project/Area Number |
15K07371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Hosei University |
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Principal Investigator |
SATO Tsutomu 法政大学, 生命科学部, 教授 (70215812)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DNA組換え / 胞子形成 / 細菌 / 部位特異的組換え / 遺伝子再構築 / 枯草菌 / 細胞分化 / 遺伝子再構成 / 溶原性ファージ / DNA組換え酵素 / ウイルス / 脱分化 |
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| Outline of Final Research Achievements |
Bacillus subtilis SPβ prophage is excised from the host chromosome in a process of sporulation to reconstitute a composite spsM. As results of in vitro recombination experiments, we revealed that the site-specific recombination occurred at the position of AA in the center of recombination site “5'-ACAGATAA /AGCTGTAT-3 '” catalyzed by an integrase SprA. Furthermore, we showed that a recombination directionality factor (RDF) SprB is necessary for synaptic complex formation between SprA and substrate DNA during excision while SprB inhibits the formation during integration. We also developed a detection system for B. subtilis dedifferentiated cells from mother cells and new genome modifying tool based on the SPβ recombination function.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] An experimentally supported model of the Bacillus subtilis global transcriptional regulatory network2015
Author(s)
Arrieta-Ortiz ML, Hafemeister C, Bate AR, Chu T, Greenfield A, Shuster B, Barry SN, Gallitto M, Liu B, Kacmarczyk T, Santoriello F, Chen J, Rodrigues CD, Sato T, Rudner DZ, Driks A, Bonneau R, Eichenberger P
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Journal Title
Mol. Syst. Biol.
Volume: 11
Issue: 11
Pages: 839-839
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 枯草菌を用いた水質浄化の試み2016
Author(s)
藤澤剛士、熊澤慶美、鈴木祥太、安部公博、佐藤勉
Organizer
第15回 微生物研究会
Place of Presentation
神奈川県藤沢市 日本大学湘南キャンパス
Year and Date
2016-11-05
Related Report
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