Molecular mechanism of amino acid signal transduction system in thermophilic bacterium
| Project/Area Number |
15K07382
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tomita Takeo 東京大学, 生物生産工学研究センター, 助教 (50447364)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アミノ酸代謝調節 / アミノ酸シグナル / グルタミン酸脱水素酵素 / 高度好熱菌 / ロイシン / アロステリック調節 / タンパク質間相互作用 / 結晶構造解析 / アミノ酸代謝 / アミノ酸シグナル応答 / トリプトファン生合成 / 活性調節 |
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| Outline of Final Research Achievements |
We found that glutamate dehydrogenase (GDH) from Thermus thermophilus interacted with a homolog of adenine phosphoribosyltransferase (APRTh). The GDH/APRTh complex was subject to allosteric activation by leucine and AMP. So far, only GDHs from mammalian have been thought to be subject to complicated allosteric regulation. We, however, found that such a complicated regulation of GDH from the organism except for mammalian. This result would lead the research of GDH regulation from various organisms and they would contribute the elucidation of complicated regulatory mechanism of human GDH, which is involved in the important cell homeostasis.
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Report
(4 results)
Research Products
(6 results)
