Chemistry and Biology of the Chromophore of a Photoprotein
| Project/Area Number |
15K07413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
KUSE Masaki 神戸大学, 農学研究科, 准教授 (40335013)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生体分子 / 生理活性 / 蛋白質 / 有機化学 / 生物発光 / 発光タンパク質 / フォラシン / デヒドロセレンテラジン / 化学合成 / 人工基質 / 発光タンパク / クロモフォア / 有機合成 / 核磁気共鳴装置 / 天然物化学 / 生物有機化学 |
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| Outline of Final Research Achievements |
Chemical and biological analyses of the chromophore of a photoprotein (pholasin) were performed in order to clarify the detailed structure of the chromophore, and the following results were obtained: 1) 13C-labled dehydrocoelenterazine (DCL) was synthesized. 2) Apo-protein was obtained by cloning and expression of the DNA of the photoprotein. 3) Non-natural DCL analogs, which gave more active substance than natural DCL, were designed and synthesized. From these results, the further progress of this research will be expected.
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Report
(4 results)
Research Products
(15 results)
