| Project/Area Number |
15K07484
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
OKU Hirosuke 琉球大学, 熱帯生物圏研究センター, 教授 (10177165)
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| Co-Investigator(Kenkyū-buntansha) |
稲福 征志 琉球大学, 熱帯生物圏研究センター, 助教 (90457458)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イソプレン / 熱帯樹木 / 温度変化 / シグナリング / シグナル伝達 / 温度依存性 / Isoprene / molecular mechanism / regulation / emission / イソプレン放出 / 調節機構 / 放出制御 / MEP経路 / 遺伝子発現 |
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| Outline of Final Research Achievements |
Isoprene emitted from terrestrial vegetation plays an important role in tropospheric chemistry. In order to clarify the temperature dependent molecular regulation of isoprene emission, we carried out a comprehensive analysis of the RNA-seq data set by using correlation analysis, network constructions and co-expression analysis. Results revealed a very close association of isoprene emission and IspS gene to genes in the plant hormone signal transduction pathway and circadian clock elements. Transcript levels of 29 genes in these pathways were validated by qRT-PCR. The upstream promoter region of IspS was also cloned and several putative cis-regulatory elements identified were consistent with transcriptional regulation in response to light, heat, temperature, plant hormones and circadian rhythmicity
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