Expoloration of temperature sensing and signaling mechanism targeting isoprene emission

• Project/Area Number: 15K07484
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: University of the Ryukyus
• Principal Investigator: OKU Hirosuke 琉球大学, 熱帯生物圏研究センター, 教授 (10177165)
• Co-Investigator(Kenkyū-buntansha): 稲福 征志 琉球大学, 熱帯生物圏研究センター, 助教 (90457458)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: イソプレン / 熱帯樹木 / 温度変化 / シグナリング / シグナル伝達 / 温度依存性 / Isoprene / molecular mechanism / regulation / emission / イソプレン放出 / 調節機構 / 放出制御 / MEP経路 / 遺伝子発現

Outline of Final Research Achievements

Isoprene emitted from terrestrial vegetation plays an important role in tropospheric chemistry. In order to clarify the temperature dependent molecular regulation of isoprene emission, we carried out a comprehensive analysis of the RNA-seq data set by using correlation analysis, network constructions and co-expression analysis. Results revealed a very close association of isoprene emission and IspS gene to genes in the plant hormone signal transduction pathway and circadian clock elements. Transcript levels of 29 genes in these pathways were validated by qRT-PCR. The upstream promoter region of IspS was also cloned and several putative cis-regulatory elements identified were consistent with transcriptional regulation in response to light, heat, temperature, plant hormones and circadian rhythmicity

Research Products

• [Journal Article] Triterpenoid modulates the salt tolerance of lanosterol synthase deficient Saccharomyces cereviciae, GIL77 (2018)
  • Author(s): Inafuku M., Basyuni M., Oku H.
  • Journal Title: Saudi Journal of Biological Sciences Volume: 25 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Molecular characterization of cytosolic cysteine synthase in Mimosa pudica. (2017)
  • Author(s): Rashid MH., Iwasaki H., Oogai S., Fukuta M., Parveen S., Hossain MA., Anai T., Oku H.
  • Journal Title: Journal of Plant Research Volume: 131 Pages: 319-329
  • Peer Reviewed
• [Journal Article] Distribution, occurrence, and cluster analysis of new polyprenyl acetones and other polyisoprenoids from North Sumatran mangroves (2017)
  • Author(s): Basyuni M., Sagami H., Baba S., Oku H.
  • Journal Title: Dendrobiology Volume: 78 Pages: 18-31
  • Peer Reviewed
• [Journal Article] Temperature controls on the basal emission rate of isoprene in a tropical tree Ficus septica: exploring molecular regulatory mechanisms (2016)
  • Author(s): Mutanda I, Inafuku M, Saitoh S, Iwasaki H, Fukuta M, Watanabe K, Oku H
  • Journal Title: Plant Cell Environ Volume: 39 Pages: 2260-2275
  • DOI: 10.1111/pce.12797
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gene expression analysis of disabled and re-induced isoprene emission by the tropical tree Ficus septica before and after cold ambient temperature exposure (2016)
  • Author(s): Mutanda I, Saitoh S, Inafuku M, Aoyama H, Takamine T, Satou K, Akutsu M, Teruya K, Tamotsu H, Shimoji M, Sunagawa H, Oku H
  • Journal Title: Tree Physiol Volume: 36 Pages: 873-882
  • DOI: 10.1093/treephys/tpw032
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Parameterization of G-93 isoprene emission formula for tropical trees Casuarina equisetifolia and Ficus septica (2016)
  • Author(s): Ishmael Mutanda, Masashi Inafuku, Hironori Iwasaki, Seikoh Saitoh, Masakazu Fukuta, Keiichi Watanabe, Hirosuke Oku
  • Journal Title: Atmospheric Environment Volume: 141 Pages: 287-296
  • DOI: 10.1016/j.atmosenv.2016.06.052
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 熱帯樹木のイソプレン放出制御におけるイソプレン合成酵素の生理学的意義 (2017)
  • Author(s): 比嘉拓也、稲福征志、屋宏典
  • Organizer: 日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会
• [Presentation] An iterative procedure to optimize predictions of isoprene emissions from tropical trees (2016)
  • Author(s): ムタンダ イシマエル、稲福征志、岩崎公典、屋宏典
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄県宜野湾市沖縄コンベンションセンター
• [Presentation] Regulation of isoprene emission in Ficus septica, a tropical tree, during changes in environmental temperature (2015)
  • Author(s): Ishmael Mutanda, Yu Kameshima, Masashi Inafuku, Seikoh Saitoh, Hirosuke Oku
  • Organizer: TERPNET 2015
  • Place of Presentation: Vancouver, BC, CA
  • Year and Date: 2015-06-01
  • Int'l Joint Research