Evaluation of toxicity of age and growth of ciguatera poison fish in the Ryukyu Archipelago

• Project/Area Number: 15K07556
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: University of the Ryukyus
• Principal Investigator: TACHIHARA Katsunori 琉球大学, 理学部, 准教授 (70264471)
• Co-Investigator(Renkei-kenkyūsha): OHSHIRO Naoya 国立医薬品食品衛生研究所, 食品衛生管理部, 第二室長 (20507032)
• Research Collaborator: HAYASHIDA Noriyuki ; NISHIMURA Mio ; ITO Mami
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: シガテラ中毒 / バラフエダイ / バラハタ / ドクウツボ / 琉球列島 / 年齢と成長 / シガトキシン、 / 年齢 / 成長 / シガトキシン / オジロバラハタ / シガテラ毒 / 宮古諸島

Outline of Final Research Achievements

We analyzed the age and growth of Lutjanus bohor, Valiola louti, V. albimarginata and Gymnothrax javanicus in the Ryukyu Archipelago and analyzed the content of ciguatoxine in each age and body weight. As a result, lifespan was 79 years old: L. bohor, 20 years old: V. louti, 15 years old: V. albimarginata, and 25 year old: G. javanicus. In all species, the tendency of ciguatoxin content tended to be higher as for older fish and larger fish. In
L. bohor, virulent individuals appeared when the body length was 500 mm, the body weight was 4 kg, aged 20 or over, and those of the Miyako Islands showed particularly high values. In V. louti and V. albimarginata, virulent individuals were only one individual. In G. javanicus, virulent individuals did not appear and it was judged that there was little risk of developing intoxication unless you intake a large amount.

Research Products

• [Presentation] ドクウツボ筋肉および肝臓のシガトキシン類分析． (2018)
  • Author(s): 大城 直雅・大久保 博英・伊藤 茉美・國吉 杏子・小島 尚・立原 一憲・朝倉 宏・安元 健．
  • Organizer: 日本食品化学学会 第24回 総会・学術大会．
• [Presentation] LC-MS/MSによるドクウツボのシガトキシン類分析. (2017)
  • Author(s): 大久保博英・伊藤茉美・國吉杏子・小島尚・立原一憲・朝倉宏・安元健・大城直雅.
  • Organizer: 第113回 日本食品衛生学会学術講演会.
• [Presentation] 琉球列島におけるウツボ科魚類の年齢と成長． (2017)
  • Author(s): 伊藤茉美・立原一憲．
  • Organizer: 2017年度日本魚類学会
• [Presentation] シガテラ毒魚オジロバラハタの年齢と成長、成熟、食性 (2016)
  • Author(s): 西村美桜・長沢寛弥・國吉杏子・小西良子・大城直雅・立原一憲
  • Organizer: 2016年度日本魚類学会年会
  • Place of Presentation: 岐阜県岐阜市岐阜大学
  • Year and Date: 2016-09-23
• [Presentation] バラフエダイ筋肉のLC-MS/MS法によるCTXs類分析． (2015)
  • Author(s): 大野祐美・風間美保・國吉杏子・林田宜之・佐久川さつき・立原一憲・小島尚・安元健・大城直雅.
  • Organizer: 第110回日本食品衛生学会（京都）
  • Place of Presentation: 京都パルスプラザ
  • Year and Date: 2015-10-29
• [Presentation] シガテラ毒魚バラハタの年齢と成長，成熟，食性． (2015)
  • Author(s): 西村美桜・渡邉美遥・小西良子・國吉杏子・大城尚雅・立原一憲．
  • Organizer: 2015年度日本魚類学会年会（近畿大学）
  • Place of Presentation: 近畿大学
  • Year and Date: 2015-09-04
• [Presentation] LC-MS/MSを用いたバラフエダイ筋肉のシガトキシン類分析の検討. (2015)
  • Author(s): 大野祐美・風間美保・國吉杏子・佐久川さつき・林田宜之・立原一憲・小島尚・安元健・大城直雅.
  • Organizer: 第25回体力・栄養・免疫学会大会 (帝京大学)．
  • Place of Presentation: 帝京大学
  • Year and Date: 2015-08-22
• [Presentation] 4C-MS analysis of ciguatoxins in fish and other marine organisms collected off the coast of Japan. (2015)
  • Author(s): Oshiro, N., S. Sakugawa, K. Kuniyoshi, R. Murata1, K. Tachihara, T.Yasumoto.
  • Organizer: Fifth Joint Symposium and AOAC Task Force Meeting Marine & Freshwater Toxins Analysis （Spain）．
  • Place of Presentation: スペイン
  • Year and Date: 2015-06-14
  • Int'l Joint Research