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The fish anesthetic-to reduce pain-

Research Project

Project/Area Number 15K07562
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionTokyo University of Agriculture

Principal Investigator

Matsubara Hajime  東京農業大学, 生物産業学部, 教授 (50459715)

Co-Investigator(Kenkyū-buntansha) 渡邉 研一  東京農業大学, 生物産業学部, 教授 (20426315)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords魚類麻酔 / 炭酸ガス / オイゲノール / ストレス / 苦痛 / 炭酸ガス麻酔 / 淡水魚 / 海水魚 / 化学的麻酔 / 物理的麻酔 / 回遊魚 / ストレスフリー
Outline of Final Research Achievements

In the present study, we investigated the fish anesthetic to reduce pain, to establish the efficient aquaculture. As the anesthetic effect of published complete genome sequences species, Tilapia and Fugu,using the solid carbon dioxide blowing tablet and the over the counter eugenol, the level of stress marker, glucose etc, are low in the high density of solid carbon dioxide blowing tablet. The high density of solution of carbon dioxide in water using solid carbon dioxide blowing tablet is dependent on its volume, the water temperature and salinity. The anesthetic effect of anadromous fish using solid carbon dioxide blowing tablet are earlier working in fresh water, in contrast these of catadromous fish are earlier working in sea water.These findings suggest that the solid carbon dioxide blowing tablet might be one of suitable anesthetic for fish to reduce stress,and the anesthetic effect of diadromous fish is dependent upon the water conditions of spawning area.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (4 results) Remarks (1 results)

  • [Int'l Joint Research] Otago University(New Zealand)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] オタゴ大学(ニュージーランド)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Testing cryopreserved European eel sperm for hybridization(#IAnguilla japonica#IR ×#IA. anguilla #IR )2018

    • Author(s)
      Muller T*, Matsubara H*, Kubara Y, Horvth ,Kolics B,Taller J, Steger V, Kovacs B, Horvath L, Asturiano J. F, Penaranda D.S, Urbnyi B.
    • Journal Title

      Theriogenology

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples.2017

    • Author(s)
      Muller T,* Matsubara H,* Kubara Y, Horvth, Asturiano J. F, Urbnyi B.
    • Journal Title

      Journal of Applied Ichthyology

      Volume: - Issue: 3 Pages: 1-3

    • DOI

      10.1111/jai.13316

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chemical and physical guidance of fish spermatozoa into egg through the Microyle2017

    • Author(s)
      Yanagimachi R,Harumi T, Matsubara H, Yan W, Yuan S, Yamaha E, Arai K, Hirohashi N, Iida T, , Matsubara T, Andoh T, Vines C, Cherr G.
    • Journal Title

      Biology of reproduction

      Volume: - Issue: 4 Pages: 780-799

    • DOI

      10.1093/biolre/iox015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Neofunctionalization of androgen receptor by gain-of-function mutations in teleost fish lineage2016

    • Author(s)
      Ogino Y, Kuraku S, Ishibashi H, Miyakawa H, Sumiya E, Miyagawa S, Matsubara H, Yamada G, Baker ME, Iguchi T
    • Journal Title

      Mol Biol Evol

      Volume: 33 Issue: 1 Pages: 228-244

    • DOI

      10.1093/molbev/msv218

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 固形炭酸ガス発泡剤の魚類に対する麻酔作用2017

    • Author(s)
      永見新,松原創,西澤和也,黒沼優,荒井大輔,大倉駿介,尾形太一,加部太一郎,桑原匡基,高橋優太,山仲舞,園田武,近藤昌和,渡邉研一
    • Organizer
      日本魚類学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 魚類精子の卵門侵入について2017

    • Author(s)
      松原創,春見達郎,永見新,西澤和也,黒沼優,小林寛,鈴木文弥,永沢旬平,高橋潤,園田 武,近藤昌和,横田高士,中野美和,市村政樹,柳町隆造
    • Organizer
      日本魚類学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Sperm entry into the micropyle of fish eggs2017

    • Author(s)
      Matsubara H, Harumi T, Nagami A, Kondo M, Takahashi M, Nishizawa K, Kuronuma Y, Ishii K, Tamura S, Tochimoto J, Yamazaki K, Watanabe M, Takamichi A, Ichimura M,Yanagimachi R
    • Organizer
      The 32th International Symposium on Okhotsk sea and ice
    • Place of Presentation
      紋別市博物館(北海道、紋別市)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] オホーツク海域の海産魚の精子の卵門進入の比較2016

    • Author(s)
      春見達郎, 松原創,市村政樹, 林要喜知, 柳町隆造
    • Organizer
      2016年度日本動物学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄、宜野湾市)
    • Year and Date
      2016-11-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 北海道網走地方及び石川県能登島地方におけるクローンドジョウの出現とその野生集団との遺伝的関係2016

    • Author(s)
      稲葉倫子,石山尚樹,松原創,藤本貴史,荒井克俊
    • Organizer
      2016年度日本水産学会北海道支部大会
    • Place of Presentation
      北海道大学(北海道、函館市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of carbon dioxide gas as fish anesthetic2016

    • Author(s)
      Hajime Matsubara,Aya Takada,Megumu Takahashi, Masakazu Kondoh, Arata Nagami,Yujiro Naka,Ryohei Yanagida, Kenta Asai, Kenta Tsunoda, Yoshinori Shiga, Ken-ichi Watanabe
    • Organizer
      The 31th International Symposium on Okhotsk sea and ice
    • Place of Presentation
      Mombetsu city ,Hokkaido
    • Year and Date
      2016-02-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Tinkering with induced spawning protocols can sustained-release androgen implants enhance the outcomes of fertility treatment in female eels?2016

    • Author(s)
      2. Lokman PM, Matsubara H, Divers SL, Damsteegt EL, Di Biase A, Setiawan AN, Wylie MJ, Thomson Laing G, Mordenti O, Todo T, Ijiri S, Adachi S.
    • Organizer
      Symposium of Towards reproduction of eel in captivity to support sustainable aquaculture (part of the EELRIC workshop)
    • Place of Presentation
      Wageningen University(Wageningen,Netherland)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] カワヤツメ種苗生産の試み2016

    • Author(s)
      松原創,高田彩,永見新,高橋潤,近藤昌和,町田善康,西澤和也,園田武,渡邉研一
    • Organizer
      2016年度日本魚類学会
    • Place of Presentation
      岐阜大学(岐阜、岐阜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 固形炭酸ガス発泡剤の麻酔効果2015

    • Author(s)
      松原創、高田彩、高橋潤、舛田稜平、永見新、中裕次郎、角田健太、志賀喜典、浅井健太、渡邉研一
    • Organizer
      日本増殖学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] Aquaculture in Japan. Can anesthesia by carbon dioxide be useful to aquaculture?2015

    • Author(s)
      Hajime Matsubara
    • Organizer
      New zealand Aquaculture research symposium 2015
    • Place of Presentation
      Newzealand,Otago University
    • Year and Date
      2015-11-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 2018年臨時増刊号2018

    • Author(s)
      渡邉研一, 松原創
    • Total Pages
      5
    • Publisher
      緑書房
    • Related Report
      2017 Annual Research Report
  • [Book] オホーツク海域における海洋資源の付加価値向上をめざして(アクアバイオ学概論)2016

    • Author(s)
      松原創
    • Total Pages
      16
    • Publisher
      生物研究社
    • Related Report
      2016 Research-status Report
  • [Book] Blue cod on the menu? (NZ Aquaculture 2016 March /April)2016

    • Author(s)
      Wylie M, Ou Z, Matsubara H, Lokman M.
    • Total Pages
      2
    • Publisher
      NZ Aquaculture
    • Related Report
      2016 Research-status Report
  • [Book] 安全安心安価簡便な魚類麻酔薬の開発(月刊養殖2016年4月号)2016

    • Author(s)
      松原創、永見新、高橋潤、高田彩、渡邉研一
    • Total Pages
      3
    • Publisher
      緑書房
    • Related Report
      2016 Research-status Report
  • [Remarks] 水圏生産科学研究室ブログ

    • URL

      http://nodai.cc-town.net/modules/nmblog/categories.php?mode=show&category=25

    • Related Report
      2016 Research-status Report 2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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