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Studies on the immunochromatography for detecting antibody to major zoonoses and infectious diseases among laboratory rat and mouse.

Research Project

Project/Area Number 15K07717
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionHokkaido University

Principal Investigator

ARIKAWA Jiro  北海道大学, 大学院医学研究院, 特任教授 (10142704)

Co-Investigator(Kenkyū-buntansha) 土佐 紀子  北海道大学, 医学研究院, 助教 (20312415)
高倉 彰  公益財団法人実験動物中央研究所, 事業部門, 理事 (60167484)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsイムノクロマト / 実験動物 / モニタリング / 人獣共通感染症 / 血清診断 / 動物由来感染症 / 微生物モニタリング / SPF / マウス / ラット
Outline of Final Research Achievements

Immunochromatography test for detecting antibody against causative agents of diseases of laboratory rat (Sendai virus, Tyzzer’s disease, Mycoplasma, sialodacryoadenitis virus, hemorrhagic fever with renal syndrome) and laboratory mouse (mouse hepatitis, Sendai virus, Tyzzer’s disease, Mycoplasma) was developed by using extracted antigen or virus particle as antigens and Protein A labeled colloidal gold particle. Results with immune serum and rat and mouse sera collected from multiple animal facilities showed the sufficient specificity and sensitivity. Further, this immunochromatography test is available with diluted blood specimens. These results indicated that this immunochromatography test is rapid and sensitive serodiagnostic tool among laboratory mouse and rats.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016

All Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] マウス主要感染症血清モニタリング用イムノクロマト法の開発2017

    • Author(s)
      土佐紀子、吉松組子、石田智子、林元展人、塩川愛絵、高倉彰、有川二郎
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] ラット・マウス主要感染症血清モニタリング用多項目イムノクロマト法の開発2017

    • Author(s)
      土佐紀子、石田智子、吉松組子、林元展人、高倉彰、有川二郎
    • Organizer
      第160回日本獣医学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Single and multiplex immunochromatographic test for diagnosis of majar infections in laboratory rats2016

    • Author(s)
      Noriko Tosa, Kumiko Yoshimatsu, Tomoko Ishida, Nobuhito Hayashimoto, Kanae Shiokawa, Akira Takakura, Jiro Arikawa
    • Organizer
      The 11th Japan-China International Conference of Virology
    • Place of Presentation
      琴弾荘、香川・観音寺市
    • Year and Date
      2016-07-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット主要感染症モニタリング用イムノクロマト法の開発2016

    • Author(s)
      土佐紀子、吉松組子、石田智子、林元展人、塩川愛絵、高倉彰、有川二郎
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール、神奈川・川崎市
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
  • [Presentation] ラット主要感染症モニタリング用イムノクロマト法の開発2016

    • Author(s)
      土佐紀子、吉松組子、石田智子、林元展人、塩川愛絵、高倉彰、有川二郎
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール、神奈川・川崎市
    • Year and Date
      2016-05-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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