Trials to improve and establish animal and human noroviruses and sapoviruses in cultured cells

• Project/Area Number: 15K07734
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Oka Tomoichiro 国立感染症研究所, ウイルス第二部, 主任研究官 (50356242)
• Co-Investigator(Kenkyū-buntansha): 高木 弘隆 国立感染症研究所, バイオセーフティ管理室, 研究員 (00332362)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: カリシウイルス / ノロウイルス / サポウイルス / リバースジェネティクス / 胆汁酸 / 感受性細胞 / ブタサポウイルス / ネコカリシウイルス / マウスノロウイルス

Outline of Final Research Achievements

We tested various cells and culture conditions to grow murine norovirus, human noroviruses, porcine sapovirus, and human sapoviruses to test the possibility of the virus propagation. During the study, an additional porcine sapovirus susceptible cell line was identified. Furthermore, several cells were susceptible for human norovirus or sapoviruses, because of detectable increase of viral RNA levels. Most of theses viruses and cells were required bile acid for virus propagation.Plasmid-based reverse genetics constructs were also applied for porcine sapovirus and feline calicivirus as an new molcular tool for virus production.

Research Products

• [Journal Article] Attempts to grow human noroviruses, a sapovirus, and a bovine norovirus in vitro (2018)
  • Author(s): Oka Tomoichiro、Stoltzfus Garrett T.、Zhu Chelsea、Jung Kwonil、Wang Qiuhong、Saif Linda J.
  • Journal Title: PLOS ONE Volume: 13 Issue: 2 Pages: 1-15
  • DOI: 10.1371/journal.pone.0178157
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First Complete Genome Sequences of Human Sapovirus Strains Classified as GI.3, GI.4, GI.6, GI.7, and GII.7 (2018)
  • Author(s): Oka Tomoichiro、Iritani Nobuhiro、Okada Mineyuki、Ogawa Tomoko、Iizuka Setsuko、Tatsumi Chika、Harada Seiya、Haga Kei、Doan Yen Hai
  • Journal Title: Genome Announcements Volume: 6 Issue: 12
  • DOI: 10.1128/genomea.00168-18
  • Peer Reviewed / Open Access
• [Journal Article] A Proposal for a Structural Model of the Feline Calicivirus Protease Bound to the Substrate Peptide under Physiological Conditions (2017)
  • Author(s): Yokoyama Masaru、Oka Tomoichiro、Takagi Hirotaka、Kojima Hirotatsu、Okabe Takayoshi、Nagano Tetsuo、Tohya Yukinobu、Sato Hironori
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 1383-1383
  • DOI: 10.3389/fmicb.2017.01383
  • Peer Reviewed / Open Access
• [Journal Article] First complete genome sequences of genogroup V, genotype 3 porcine sapoviruses: common 5′-terminal genomic feature of sapoviruses (2017)
  • Author(s): Oka Tomoichiro、Doan Yen Hai、Shimoike Takashi、Haga Kei、Takizawa Takenori
  • Journal Title: Virus Genes Volume: 53 Issue: 6 Pages: 848-855
  • DOI: 10.1007/s11262-017-1481-8
  • Peer Reviewed
• [Journal Article] Antiviral effect of theaflavins against caliciviruses (2017)
  • Author(s): Ohba, M., Oka, T., Ando, T., Arahata, S., Ikegaya, A., Takagi, H., Ogo, N., Zhu, C., Owada, K., Kawamori, F., Wang, Q., Saif, L. J. and Asai, A.
  • Journal Title: The Journal of Antibiotics Volume: 70 Issue: 4 Pages: 443-447
  • DOI: 10.1038/ja.2016.128
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genetic Characterization of Rare Genotype GII.5 Sapovirus Strain Detected from a Suspected Food-Borne Gastroenteritis Outbreak among Adults in Japan in 2010 (2017)
  • Author(s): Oka T, Doan YH, Haga K, Mori K, Ogawa T, Yamazaki A.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 2 Pages: 223-224
  • DOI: 10.7883/yoken.JJID.2016.468
  • NAID: 130005475417
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic Characterization and Classification of Human and Animal Sapoviruses (2016)
  • Author(s): Oka T, Lu Z, Phan T, Delwart EL, Saif LJ, Wang Q.
  • Journal Title: PLOS ONE Volume: 11 Issue: 5 Pages: e0156373-e0156373
  • DOI: 10.1371/journal.pone.0156373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanism of Cell Culture Adaptation of an Enteric Calicivirus, the Porcine Sapovirus Cowden Strain (2016)
  • Author(s): Zhongyan Lu, Masaru Yokoyama, Ning Chen, Tomoichiro Oka, Kwonil Jung, Kyeong-Ok Chang,Thavamathi Annamalai, Qiuhong Wang, Linda J. Saif
  • Journal Title: Journal of Virology Volume: 90 Issue: 3 Pages: 1345-1358
  • DOI: 10.1128/jvi.02197-15
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Epidemics of GI.2 Sapovirus in Gastroenteritis Outbreaks During 2012-2013 in Osaka City, Japan (2016)
  • Author(s): Nobuhiro Iritani, Seiji P. Yamamoto, Niichiro Abe, Hideyuki Kubo, Tomoichiro Oka, and Atsushi Kaida
  • Journal Title: Journal of Medical Virology Volume: 88 Issue: 7 Pages: 1187-1193
  • DOI: 10.1002/jmv.24451
  • Peer Reviewed
• [Journal Article] Nonstructural protein p39 of feline calicivirus suppresses host innate immune response by preventing IRF-3 activation (2016)
  • Author(s): Yo Yumiketa, Takanori Narita, Yosuke Inoue, Go Sato, Wataru Kamitani, Tomoichiro Oka, Kazuhiko Katayama, Takemasa Sakaguchi, Yukinobu Tohya
  • Journal Title: Veterinary Microbiology Volume: 185 Pages: 62-67
  • DOI: 10.1016/j.vetmic.2016.02.005
  • Peer Reviewed
• [Journal Article] Discovery and Synthesis of Heterocyclic Carboxamide Derivatives as Potent Anti-norovirus Agents (2016)
  • Author(s): Mai Ohba, Tomoichiro Oka, Takayuki Ando, Saori Arahata, Asaka Ikegaya, Hirotaka Takagi, Naohisa Ogo, Kazuhiro Owada, Fumihiko Kawamori, Qiuhong Wang, Linda J. Saif, Akira Asai
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 64 Issue: 5 Pages: 465-475
  • DOI: 10.1248/cpb.c16-00001
  • NAID: 130005148748
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Attempts to grow human noroviruses and a sapovirus in vitro (2017)
  • Author(s): 2.Tomoichiro Oka, Garrett T Stoltzfus, Chelsea Zhu, Kwonil Jung, Qiuhong Wang, Linda J. Saif
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] 培養可能なカリシウイルスの新規plasmid-basedリバースジェネティクス系の構築 (2016)
  • Author(s): 岡 智一郎、高木弘隆、遠矢幸伸
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] Discovery of novel anti-calicivirus agents. (2016)
  • Author(s): Ohba, M., Oka, T., Ando, T., Arahata, S., Ikegaya, A., Takagi, H., Ogo, N., Zhu, C., Owada, K., Kawamori, F., Wang, Q., Saif, L. J. and Asai, A.
  • Organizer: The 3rd International Conference on Pharma-Food (ICPF 2016)
  • Place of Presentation: 静岡
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] Molecular Epidemiology of Human Sapoviruses (2016)
  • Author(s): Oka, T.
  • Organizer: The 6th International Calicivirus Conference
  • Place of Presentation: Savannah, GA, USA.
  • Year and Date: 2016-10-09
  • Int'l Joint Research / Invited
• [Presentation] Discovery and synthesis of heterocyclic carboxamide derivatives as potent anti-norovirus agents. (2016)
  • Author(s): Ohba, M., Oka, T., Ando, T., Arahata, S., Ikegaya, A., Takagi, H., Ogo, N., Zhu, C., Owada, K., Kawamori, F., Wang, Q., Saif, L. J. and Asai, A.
  • Organizer: The 6th International Calicivirus Conference
  • Place of Presentation: Savannah, GA, USA.
  • Year and Date: 2016-10-09
  • Int'l Joint Research
• [Presentation] ネコカリシウイルスプロテアーゼの基質特徴を有する非ペプチド性低分子化合物の抗ウイルス活性 (2016)
  • Author(s): 岡 智一郎、高木 弘隆、横山 勝、小島 宏建、岡部 隆義、長野 哲雄、佐藤 裕徳
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: 神奈川県横浜市みなとみらい (パシフィコ横浜)
  • Year and Date: 2016-03-28
• [Presentation] 抗ノロウイルス活性を有する低分子化合物の探索 (2016)
  • Author(s): 大場舞, 岡智一郎, 安藤隆幸, 荒畑沙織, 池ヶ谷朝香, 高木弘隆, 小郷尚久, 小和田和宏, 川森文彦, 浅井章良
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: 神奈川県横浜市みなとみらい (パシフィコ横浜)
  • Year and Date: 2016-03-28