Development of a stimulus-responsive amino acid that enables functional control of peptides and proteins
| Project/Area Number |
15K07858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHIGENAGA Akira 徳島大学, 大学院医歯薬学研究部(薬学域), 講師 (10423394)
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| Co-Investigator(Renkei-kenkyūsha) |
OTAKA Akira 徳島大学, 大学院医歯薬学研究部(薬学域), 教授 (20201973)
INOKUMA Tsubasa 徳島大学, 大学院医歯薬学研究部(薬学域), 助教 (40541272)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | トリメチルロック / 機能制御 / ペプチド / タンパク質 / 刺激応答 / ラクトン化 / アミド結合切断 / ペプチド結合切断 / 活性制御 / 刺激応答型アミノ酸 |
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| Outline of Final Research Achievements |
A stimulus-responsive amino acid, which induces stimulus-triggered amide/peptide bond cleavage, enables functional control of peptides and proteins. In this project, we challenged to accelerate the amide bond cleavage of the stimulus-responsive unit that should be the bases for practical stimulus-responsive amino acids. We first established a fluorescence-based system to easily monitor the amide bond cleavage. Then, several new stimulus-responsive units were synthesized and their kinetics of the amide bond cleavage was evaluated based on the fluorescence monitoring. Finally, we successfully found out a new stimulus-responsive unit, its amide bond cleavage is significantly accelerated under acid conditions.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Labelling of endogenous target protein via N-S acyl transfer-mediated activation of N-sulfanylethylanilide2016
Author(s)
Denda, M.; Morisaki, T.; Kohiki, T.; Yamamoto, J.; Sato, K.; Sagawa, I.; Inokuma, T.; Sato, Y.; Yamauchi, A.; Shigenaga, A.*; Otaka, A.*
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Journal Title
Org. Biomol. Chem.
Volume: 14
Issue: 26
Pages: 6244-6251
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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