Molecular mechanism of ciliary vesicle formation in mammalian cells
| Project/Area Number |
15K07931
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Kobayashi Tetsuo 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80433994)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 一次繊毛 / 繊毛小胞 |
|---|
| Outline of Final Research Achievements |
By comprehensive proteome screen, we identified novel small GTPase Rab-like 3 (Rabl3) as a putative binding partner of Rab11 which is involved in ciliary vesicle formation. We showed that Rabl3 physically interacts with Rab11. We also found that Rabl3 localizes to centrioles by immunofluorescence analysis in RPE1 cells. Furthermore, we observed that Rabl3 depletion causes decrease of primary cilia formation in RPE1 cells.
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Report
(4 results)
Research Products
(12 results)
