Functional significance of the delta-protocadherins in the development of the CNS and the rostral structures

• Project/Area Number: 15K08128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Gunma University
• Principal Investigator: Murakami Tohru 群馬大学, 大学院医学系研究科, 准教授 (10239494)
• Research Collaborator: Tajika Yuki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発生学・形態形成学 / 細胞接着因子 / プロトカドヘリン / ゼブラフィッシュ / 3D画像解析 / ブロックフェースイメージング

Outline of Final Research Achievements

The purpose of this project was to elucidate the significance of the delta-protocadherins in the development and maintenance of the CNS and rostral structures. High throughput 3D analysis technology was developed to make structural analyses of the embryos though adult animals, which successfully yielded detailed 3D images of specimens of several millimeters to centimeters. There were encountered some difficulties on a development of technologies for higher resolution 3D images, which prompted further pursuit for the best practices of imaging and molecular staining technologies.

Research Products

• [Journal Article] Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung. (2018)
  • Author(s): Ikezawa M, Tajika Y, Ueno H, Murakami T, Inoue N, Yorifuji H.
  • Journal Title: Developmental Dynamics Volume: 247 Issue: 5 Pages: 754-762
  • DOI: 10.1002/dvdy.24618
  • Peer Reviewed / Open Access
• [Journal Article] A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging (2017)
  • Author(s): Tajika Yuki、Murakami Tohru、Iijima Keiya、Gotoh Hiroki、Takahashi-Ikezawa Maiko、Ueno Hitoshi、Yoshimoto Yuhei、Yorifuji Hiroshi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 3645-3645
  • DOI: 10.1038/s41598-017-03900-9
  • Peer Reviewed / Open Access
• [Journal Article] 臨床への解剖学 (2016)
  • Author(s): 村上 徹
  • Journal Title: 日本生理学学会雑誌 Volume: 78
  • Peer Reviewed / Open Access
• [Presentation] 胎生期マウスの2Dおよび3D形態解析 (2017)
  • Author(s): 多鹿友喜、村上徹、池澤麻衣子、上野仁之、依藤宏
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] Visible Fish Danio rerio (2016)
  • Author(s): 村上 徹, 多鹿友喜, 上野仁之, 池澤麻衣子, 依藤 宏
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-30
• [Presentation] Serial block-face imaging system for both 3D morphology and section histology (2015)
  • Author(s): Yuki Tajika, Tohru Murakami, Maiko Takahashi, Hitoshi Ueno, Hiroshi Yorifuji
  • Organizer: ASCB Annual Meeting
  • Place of Presentation: Moscone Center (San Diego, USA)
  • Year and Date: 2015-12-12
• [Remarks] ポートフォリオ 群馬大学大学院医学系研究科機能形態学
  • URL: http://pandora.med.gunma-u.ac.jp/portfolio/
• [Remarks] Protocols
  • URL: http://anatomy.med.gunma-u.ac.jp/protocols/