Analysis of the autocrine DNA fragmentation mechanisms in mouse intestinal intraepithelial lymphocytes
| Project/Area Number |
15K08146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tohoku Fukushi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
尾形 雅君 東北医科薬科大学, 薬学部, 講師 (50311907)
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マウス / 小腸 / 上皮細胞 / γδ-IELs / TNFα / DNA断片化 / パーフォリン / グランザイム / マクロファージ / 粘膜固有層 / IEL / DNA修復 / Granzyme B / Perforin |
|---|
| Outline of Final Research Achievements |
γδ-IELs appear to readily and rapidly respond, though at only one time, to various stimulations. They could be recognized as one-shot responders and disposable cells of the defense system present in the front line intestinal epithelium. Activated γδ-IELs not only induced DNA fragmentation in villus epithelial cells (IECs); it induced identical DNA fragmentation in the γδ-IELs themselves (the autocrine DNA fragmentation). The DNA fragmentation as well as the DNA repair thereafter should thus be interpreted in a more generalized phenomenon. The transient diarrhea due to the detachment of IECs induced by activated γδ-IELs was shown to be caused by TNFα derived from macrophages located in the lamina propria, suggesting the involvement of the γδ-IELs in the elimination of IECs in a defense system, for example, when IECs are infected with pathogenic viruses.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Inhibition of fatty acid synthase decreases expression of stemness markers in glioma stem cells.2016
Author(s)
Yasumoto Y., Miyazaki H., Vaidyan L.K., Kagawa Y., Ebrahimi M., Yamamoto Y., Ogata M., Katsuyama Y., Sadahiro H., Suzuki M., & Owada Y.
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Journal Title
PLoS One
Volume: 11(1)
Issue: 1
Pages: 11-11
DOI
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Peer Reviewed / Open Access
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[Presentation] 人体の構造と機能2016
Author(s)
尾形雅君
Organizer
第10回 基礎ゼミ・展開ゼミ FDワークショップ
Place of Presentation
東北大学川内キャンパス（宮城県仙台市）
Year and Date
2016-11-10
Related Report
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[Book] 新膵臓病学2017
Author(s)
尾形雅君 （下瀬川徹編）
Total Pages
528
Publisher
南光堂
Related Report
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