| Project/Area Number |
15K08181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
KAMEYAMA Masaki 鹿児島大学, 医歯学総合研究科, 客員研究員 (60150059)
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| Co-Investigator(Kenkyū-buntansha) |
徐 建軍 鹿児島大学, 医歯学域医学系, 講師 (10581689)
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| Co-Investigator(Renkei-kenkyūsha) |
MINOBE Etsuko 鹿児島大学, 医歯学域医学系, 講師 (00448581)
GAO Qinghua 鹿児島大学, 医歯学総合研究科, 特任研究員 (60813524)
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| Research Collaborator |
MORI Tomoyuki 京都大学, 工学研究科, 准教授
HAO Liying 中国医科大学, 薬学院薬理毒理学, 教授
ZHU Tong 東北大学, 機械工程及自動化学院, 教授
LYU Liting 東北大学, 機械工程及自動化学院, 大学院生
LEI Ming 中国医科大学, 薬学院薬理毒理学, 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Caチャネル / Aキナーゼ / カルモジュリン / 心筋 / Ca2+依存性調節 / リン酸化 / Ca2+ チャネル / 心筋細胞 / イオンチャネル |
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| Outline of Final Research Achievements |
We have investigated mechanisms underlying Cav1.2 Ca2+ channel regulation. We have found that: 1) the Ca2+ channels bind with protein kinase A (PKA) and protein phosphatases even in the inside-out mode; 2) there is a new interaction between CaM-binding region in the proximal C-terminal (CT1) and a region in the distal C-terminal (CT3) of alpha1C subunit; 3) PKA phosphorylation of CT1 affects the binding not only between CaM and CT1 but also the new proximal-distal (CT1-CT3) interaction. These results suggest that PKA phosphorylation regulate the Cav1.2 channel activity, at least partially, via affecting the CaM-CT1 interaction.
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