Roles of neuron types and functions defining general framework for activation sequence of inspiratory neurons during inspiratory rhythmic bursts

• Project/Area Number: 15K08196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Hyogo Medical University
• Principal Investigator: OKE Yoshihiko 兵庫医科大学, 医学部, 助教 (50396470)
• Co-Investigator(Kenkyū-buntansha): 三分一 史和 統計数理研究所, モデリング研究系, 准教授 (30360647)
• Research Collaborator: OKU yoshitaka
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 呼吸中枢 / preBotzinger complex / 自発的同期活動 / 神経ネットワーク / 二光子顕微鏡 / カルシウムイメージング / 抑制性ニューロン

Outline of Final Research Achievements

In this study, we investigated relationships between inspiratory neuron types and activation sequence during rhythmic bursts in the pre-Botzinger complex (preBotC). In earlier phase during each rhythmic burst, any one of inspiratory neurons of which activation patterns showed small maximum cross-correlation coefficient (maxCC) with bursting pattern were activated. On the other hand, inspiratory neurons with large maxCC were activated at/after middle phase. There was no significant differences between activation timings of excitatory and inhibitory inspiratory neurons.

Academic Significance and Societal Importance of the Research Achievements

呼吸活動をはじめとする生命活動に必須な運動に関する神経ネットワークには、頑強性が要求される。本研究の成果は、呼吸中枢がもつ柔軟なネットワークダイナミクスの意義に迫る結果であり、機能的な神経ネットワークの新たなパラダイムを提供しており、新しい概念の神経マップの作成等を通じて脊髄損傷の再生医療などに貢献することが期待出来る。

Research Products

• [Int'l Joint Research] University Medical Center, Gottingen/University of Leipzig/Max Plank Institute, Gottingen(ドイツ)
• [Int'l Joint Research] ゲッティンゲン大学/Max Planck Institute/University of Leipzig(ドイツ)
• [Int'l Joint Research] Goettingen University/Clinic for Anesthesiology(ドイツ)
• [Int'l Joint Research] University of Leipzig/Carl-Ludwig-Institute for Physiology(ドイツ)
• [Journal Article] The postnatal development of ultrasonic vocalization-associated breathing is altered in glycine transporter 2-deficient mice (2019)
  • Author(s): S. Hulsmann, Y. Oke, G. Mesuret, A. T. Latal, M. G. Fortuna, M. Niebert, J. Hirrlinger, J. Fischer and K. Hammerschmidt
  • Journal Title: Journal of Physiology Volume: 597 (1) Issue: 1 Pages: 173-191
  • DOI: 10.1113/jp276976
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell type-dependent activation sequence during rhythmic bursting in the preBotzinger complex in respiratory rhythmic slices from mice (2018)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, J. Hirrlinger and S. Hulsmann
  • Journal Title: Frontiers in Physiology Volume: 9 Pages: 1219-1219
  • DOI: 10.3389/fphys.2018.01219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stochastic activation among inspiratory cells in the pre-Botzinger complex of the rat medulla revealed by Ca2+ imaging (2015)
  • Author(s): Y. Oke, D. Boiroux, F. Miwakeichi, Y .Oku
  • Journal Title: Neuroscience Letters Volume: 595 Pages: 12-17
  • DOI: 10.1016/j.neulet.2015.04.003
  • Peer Reviewed
• [Presentation] Cell type-based activation timing and order in the sequence in the pre-Botzinger complex (2019)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, J. Hirrlinger and S. Hulsmann
  • Organizer: 9th Congress of the Federation of the Asian and Oceanian Physiological Societies (FAOPS) in conjunction with the 96th Annual Meeting of the Physiological Society of Japan
  • Int'l Joint Research
• [Presentation] Glycine transporter 2-deficient mice show an altered development of the ultrasonic vocalization-associated breathing (2019)
  • Author(s): S. Hulsmann, Y. Oke, G. Mesuret, A. T. Latal, M. G. Fortuna, M. Niebert, J. Hirrlinger, J. Fischer and K. Hammerschmidt
  • Organizer: 13th Gottingen Meeting of the German Neuroscience Society
  • Int'l Joint Research
• [Presentation] Activation timing and order in the sequence during rhythmic burst is dependent on cell type of inspiratory neurons in the pre-Botzinger complex of the mice medulla slice (2018)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, J. Hirrlinger and S. Hulsmann
  • Organizer: The 48th Annual Meeting of Society for Neuroscience (Neuroscience 2018)
  • Int'l Joint Research
• [Presentation] Spatio-temporal analysis of multi-neuronal imaging data and visualization of spontaneous neuronal activation patterns（マルチニューラルイメージングデータの時空間解析と自励的神経活動化パターンの視覚化） (2018)
  • Author(s): F. Miwakeich, Y. Oke, Y. Oku, Andreas Galka and S. Hulsmann
  • Organizer: 第41回日本神経科学大会
• [Presentation] Neuronal type-dependent stochastic activation sequence among inspiratory neurons during rhythmic burst in the pre-Botzinger complex of the mice medulla slice (2017)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, S. Besser, J. Hirrlinger and S. Huelsmann.
  • Organizer: The 47th Annual Meeting of Society for Neuroscience (Neuroscience 2017)
  • Int'l Joint Research
• [Presentation] General pattern of activation sequence among excitatory/inhibitory inspiratory neurons during rhythmic burst in the pre-Botzinger complex of the mice medulla slice (2017)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, S. Besser, J. Hirrlinger and S. Huelsmann.
  • Organizer: The 14th Oxford Conference on Modelling and Control of Breathing
  • Int'l Joint Research
• [Presentation] Detection of irregularly firing inspiratory neurons in the pre-Botzinger complex based on spatio-temporal optical imaging data analysis (2017)
  • Author(s): F. Miwakeichi, Y. Oke, Y. Oku and S. Huelsmann.
  • Organizer: The 47th Annual Meeting of Society for Neuroscience (Neuroscience 2017)
  • Int'l Joint Research
• [Presentation] Rhythmic activation patterns of excitatory/inhibitory inspiratory neurons in the pre-Botzinger complex of the mice medulla slice（マウス延髄スライス標本のpre-Botzinger complexにおける自発的同期活動を起こす興奮性ならびに抑制性の吸息性ニューロンの活性化パターン） (2017)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, S. Besser, J. Hirrlinger, S. Huelsmann
  • Organizer: 第94回日本生理学会大会（The 94th Annual Meeting of The Physiological Society of Japan）
  • Place of Presentation: アクトシティ浜松（静岡県，浜松市）
• [Presentation] Development of inhibitory inspiratory neurons in the pre-Botzinger complex of neonatal mice（発達過程における新生児マウスのPre-Botzinger complex領域における吸息性抑制ニューロン数の変化） (2016)
  • Author(s): Y. Oke, F. Miwakeichi, Y. Oku, S. Besser, J. Hirrlinger, S. Huelsmann
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-03-24
• [Presentation] A statistical mapping strategy to identify inspiratory neurons among active cells in the pre-Botzinger Complex (2016)
  • Author(s): 三分一史和、尾家慶彦
  • Organizer: データ科学シンポジウム2015 欠測データ解析とモデル選択: 生体情報 データの統計モデル
  • Place of Presentation: 大阪大学（大阪府、吹田市）
  • Year and Date: 2016-01-22
  • Invited
• [Presentation] A Statistical Mapping Strategy to Identify Inspiratory Neurons among Active Cells in the Pre-Bozinger Complex (2015)
  • Author(s): F. Miwakeich, Y. Oke, Y. Oku, Andreas Galka and S. Huelsmann
  • Organizer: 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milan,Italy
  • Year and Date: 2015-08-29
  • Int'l Joint Research
• [Presentation] Differentiation of neurons and astrocyte in brain stem by statistical image processing methods（統計的画像解析による脳幹のニューロン、アストロサイトの識別） (2015)
  • Author(s): F. Miwakeich, Y. Oke, Y. Oku, Andreas Galka and S. Huelsmann
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-29
• [Presentation] tochastic activation of inspiratory cells in the pre-Bötzinger complex with loose regularity during spontaneous inspiratory rhythmic bursts（自発的な吸息性バースト時には、pre-Bötzinger complex領域に存在する吸息性細胞は緩やかな秩序に従って活性化する） (2015)
  • Author(s): Y. Oke, D. Bouiroux, F. Miwakeichi and Y. Oku
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] A statistical strategy of optical imaging data analysis to differentiate neurons and astrocytes in the brain stem (2015)
  • Author(s): F. Miwakeich, Y. Oke, Y. Oku, Andreas Galka and S. Huelsmann
  • Organizer: Statistical Computing Asia 2015
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-07-02
  • Int'l Joint Research