| Project/Area Number |
15K08407
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
TANAKA Hideo 京都府立医科大学, 医学研究科, 教授 (60236619)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
山岡 禎久 佐賀大学, 工学(系)研究科(研究院), 准教授 (80405274)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MATSUYAMA Takaaki 昭和大学, 医学部・法医学講座, 准教授 (40349113)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 横行小管 / カルシウム / 心房筋 / 心房細動 / 不整脈 / プルキンエ線維 / 心筋梗塞 / 共焦点顕微鏡 / 膜電位 / 伝導障害 / 蛍光顕微鏡 / 摘出灌流心 / 心房リモデリング |
|---|
| Outline of Final Research Achievements |
We address the possibility that altered distribution of transverse (T) tubules contributes to genesis of cardiac arrhythmias by confocal microscopy. Compared with ventricular myocytes, atrial cells, which barely show T tubules, exhibited spatiotemporally non-uniform Ca2+ dynamics, whereas ventricular myocytes showing fine, regular distribution of the tubules had uniform Ca2+ transients. The inhomogeneous Ca2+ dynamics in the atrial cells were more evident at higher frequency of excitation. However, no definitive relevance was obtained between the T tubule distribution and arrhythmogenesis. Besides, we obtained exact relevance of inhomogeneity of Ca2+ dynamics to T-tubule distribution in Purkinje fibers in mouse infarct border zone. Together, inhomogeneous Ca2+ dynamics owing to the paucity of T tubular distribution does not directly evoke arrhythmias; however, such inhomogeneity may contribute to depressed conductivity and mechanical performances, which may lead to arrhythmogenesis.
|
