Control of Chronic Liver Diseases due to Hepatitis C Virus by Long Non-coding RNAs
Project/Area Number |
15K08492
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | Kanazawa University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | C型肝炎ウイルス / 長鎖ノンコーディングRNA / C型慢性肝炎 / ノンコーディングRNA |
Outline of Final Research Achievements |
We investigated the role of long non-coding RNAs in HCV infection. The analysis on RNA of HCV-infected cultured hepatocytes by a next generation sequencer revealed that the RNA level of one of long non-coding RNAs, HULC, increased. The increase of HULC RNA level was observed not only in HCV-infected cultured hepatocytes, but also in an HCV-infected chimpanzee and a HCV-infected mouse. The knockdown of HCV by siRNA suppressed HCV replication as well as infectious virus production in HCV-infected cultured hepatocytes. This effect seemed to occur through the suppression of a translation step among HCV life cycles. Several recent reports show that HULC has the ability to promote hepatocarcinogenesis, therefore, the result of this study suggests a mechanism of hepatocarcinogesis due to HCV infection through HULC.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Peretinoin, an Acyclic Retinoid, Inhibits Hepatitis B Virus Replication by Suppressing Sphingosine Metabolic Pathway In Vitro.2018
Author(s)
1.Murai K, Shirasaki T, Honda M, Shimizu R, Shimakami T, Nakasho S, Shirasaki N, Okada H, Sakai Y, Yamashita T, Kaneko S.
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Journal Title
International Journal of Molecular Sciences.
Volume: 19(2)
Issue: 2
Pages: 108-108
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Unexpected Replication Boost by Simeprevir for Simeprevir-resistant Variants in Genotype 1a Hepatitis C Virus2018
Author(s)
Murai K, Shimakami T, Welsch C, Shirasaki T, Liu F, Kitabayashi J, Tanaka S, Funaki M, Omura H, Nishikawa T, Suminyadorj A, Honda M, Kaneko S.
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Journal Title
Antimicrob Agents Chemother
Volume: 印刷中
Issue: 7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Peretinoin, an acyclic retinoid, inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo.2017
Author(s)
Funaki M, Kitabayashi J, Shimakami T, Nagata N, Sakai Y, Takegoshi K, Okada H, Murai K, Shirasaki T, Oyama T, Yamashita T, Ota T, Takuwa Y, Honda M, Kaneko S.
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 16978-16978
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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