Characterization of avian influenza virus variants isolated from infected patients
| Project/Area Number |
15K08497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Watanabe Yohei 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50452462)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 康夫 中部大学, 生命健康科学部, 客員教授 (00046278)
大道寺 智 京都府立医科大学, 医学(系)研究科(研究院), 講師 (80432433)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Yasuha 京都府立医科大学, 医学研究科, 博士研究員 (80793182)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ウイルス学 / 人獣共通感染症学 / 感染病態学 / インフルエンザウイルス |
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| Outline of Final Research Achievements |
Our database search of avian H5N1 influenza viruses identified 59 HA mutations that had been selected in infected patients. Using reverse genetics, we found that H5N1 variants with some of these HA mutations had increased human-type receptor specificity and increased HA membrane fusion activity, both of which are advantageous for viral replication in human airway epithelia. Furthermore, HA mutants selected during viral replication in patients were likely to have less HA stability, apparently as compensatory mechanism. Our findings showed that H5N1 viruses could rapidly adapt to growth in the human airway microenvironment by altering HA properties in infected patients and provided new insights into the human-adaptive mechanisms of avian influenza viruses.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Characterization of H5N1 influenza virus quasispecies with adaptive hemagglutinin mutations from single-virus infections of human airway cells2018
Author(s)
1.Watanabe Y**, Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Kajikawa J, Hiramatsu H, Sriwilaijaroen N, Ono T, Takagi T, Takahashi K, Shioda T, Matsumoto K, Suzuki Y, Nakaya T
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Journal Title
J Virol.
Volume: 印刷中
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses2016
Author(s)
Arai Y, Kawashita N, Daidoji T, Ibrahim MS, El-Gendy EM, Takagi T, Takahashi K, Suzuki Y, Ikuta K, Nakaya T, Shioda T, Watanabe Y
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Journal Title
PLoS Pathog.
Volume: 12
Issue: 4
Pages: e1005583-e1005583
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characterization of H5N1 Influenza Virus Variants with Hemagglutinin Mutations Isolated from Patients.2015
Author(s)
1.Watanabe Y, Arai Y, Daidoji T, Kawashita N, Ibrahim MS, El-Gendy E, Hiramatsu H, Kubota-Kokestu R, Takagi T, Murata T, Takahashi K, Okuno Y, Nakaya T, Suzuki Y, Ikuta K.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Characterization of H5N1 influenza virus with HA mutations diversified in human airway epithelial cells as minor variants.2017
Author(s)
Watanabe Y, Arai Y, Kawashita N, Daidoji T, Elgendy EM, Ibrahim MS, Kajikawa J, Hiramatsu H, Takagi T, Takahashi K, Shioda T, Suzuki Y, Nakaya T.
Organizer
第65回日本ウイルス学会学術集会
Related Report
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[Presentation] Novel polymerase gene mutations for human adaptation in clinical isolates of Avian H5N1 influenza virus in Asia.2017
Author(s)
Arai Y, Kawashita N, Hotta K, Hoang MPV, Nguyen KHL, Nguyen TC, Vuong CD, Le MQ, Soda K, Kajikawa J, Daidoji T, Nakaya T, Takagi T, Ito T, Hasebe H, Shioda T, Watanabe Y.
Organizer
第65回日本ウイルス学会学術集会
Related Report
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