| Project/Area Number |
15K08618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi University |
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Principal Investigator |
TAKEUCHI Hiroaki 高知大学, 教育研究部医療学系臨床医学部門, 講師 (90346560)
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| Co-Investigator(Kenkyū-buntansha) |
松村 敬久 高知大学, 教育研究部医療学系臨床医学部門, 教授 (10274391)
杉浦 哲朗 高知大学, 医学部附属病院, 特任教授 (50171145)
森本 徳仁 高知大学, 医学部附属病院, 臨床検査技師 (60398055)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ヘリコバクター・ピロリ / H. pylori flora / 細胞分裂制御機構 / 形態形成 / minCDE / ftsZ / 生物学的多様性 / ファージ / NO system / Coccoid / coccoid |
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| Outline of Final Research Achievements |
The machinery of cell division in H. pylori is little known. We investigated the function of Min proteins and FtsZ, and provided new insights as follows; 1. All Min proteins (C, D and E) regulated the cell elongation, 2. MinC and D regulated the division site in cells, 3. MinC regulated the Z-ring polymerization and contributed to the FtsZ stability, 4.MinD involved in nucleic occlusion system, and 5. MinE involved in the coccoid conversion at the stationary phase.
We obtained prophage-cured derivative strains from NY43 strain infected with prophage KHP30. The comparative analyses with NY43 and its prophage-cured strains provided new insights as follows; 1. Prophage induced the genetic mutations in cagA, leading to CagA disruption, 2. Prophage influenced the morphology and motility, 3. The prophage-cured derivatives could re-infect with phage, indicating that the repeated/patterned phenomena would be involved in the development of H. pylori evolution with biological polymorphisms.
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