Analyses for the unique machinery of cell division in Helicobacter pylori and pathogenesis of its associated disorders
| Project/Area Number |
15K08618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi University |
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Principal Investigator |
TAKEUCHI Hiroaki 高知大学, 教育研究部医療学系臨床医学部門, 講師 (90346560)
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| Co-Investigator(Kenkyū-buntansha) |
松村 敬久 高知大学, 教育研究部医療学系臨床医学部門, 教授 (10274391)
杉浦 哲朗 高知大学, 医学部附属病院, 特任教授 (50171145)
森本 徳仁 高知大学, 医学部附属病院, 臨床検査技師 (60398055)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ヘリコバクター・ピロリ / H. pylori flora / 細胞分裂制御機構 / 形態形成 / minCDE / ftsZ / 生物学的多様性 / ファージ / NO system / Coccoid / coccoid |
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| Outline of Final Research Achievements |
The machinery of cell division in H. pylori is little known. We investigated the function of Min proteins and FtsZ, and provided new insights as follows; 1. All Min proteins (C, D and E) regulated the cell elongation, 2. MinC and D regulated the division site in cells, 3. MinC regulated the Z-ring polymerization and contributed to the FtsZ stability, 4.MinD involved in nucleic occlusion system, and 5. MinE involved in the coccoid conversion at the stationary phase.
We obtained prophage-cured derivative strains from NY43 strain infected with prophage KHP30. The comparative analyses with NY43 and its prophage-cured strains provided new insights as follows; 1. Prophage induced the genetic mutations in cagA, leading to CagA disruption, 2. Prophage influenced the morphology and motility, 3. The prophage-cured derivatives could re-infect with phage, indicating that the repeated/patterned phenomena would be involved in the development of H. pylori evolution with biological polymorphisms.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Screening of KHP30-like prophages among Japanese Helicobacter pylori strains, and genetic analysis of a defective KHP30-like prophage sequence integrated in the genome of the H. pylori strain NY40.2016
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Takeuchi H, Sakaguchi Y, Ujihara T, Daibata M, Shimakura H, Okamoto N, Sakaguchi M, Matsuzaki S.
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Journal Title
FEMS Microbiol Lett.
Volume: 363
Issue: 16
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] VONOPRAZAN-BASED THIRD-LINE TRIPLE THERAPY IN HELICOBACTER PYLORI ERADICATION AND STUDY OF THE DIVERSITY OF ANTIMICROBIAL SUSCEPTIBILITIES2017
Author(s)
YUKARI YANO, HIROSHI MIZUTA, MASAFUMI ONO, SHO NAGANO, HISAKO TSUDA, TATSUYA KITAGAWA, MOTOI HASHIBA, NOBUTO OKAMOTO, MIZUKI KIRA, HIROAKI TAKEUCHI, YOSHIHISA MATSUMURA, TOSHIJI SAIBARA
Organizer
アジア太平洋消化器病週間
Related Report
Int'l Joint Research
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[Presentation] Screening of KHP30-like prophage in Helicobacter pylori using Japanese strains, and analysis of their fate by in silico approach2016
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Takeuchi H, Sakaguchi Y, Ujihara T, Nasukawa T, Shimakura H, Okamoto N, Sakaguchi M, Matsuzaki S
Organizer
ファージ研究会・ファージセラピー臨床応用検討会・微生物生態学会 環境ウイルス部会合同シンポジウム
Place of Presentation
神奈川県横須賀市JAMSTEC横浜本部
Year and Date
2016-10-21
Related Report
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[Presentation] Potassium-competitive acid blocker-based third-line triple therapy in Helicobacter pylori eradication and study of the diversity of antimicrobial susceptibilities2016
Author(s)
Yukari Yano, Hiroshi Mizuta, Mizuki Kira, Yoshihisa Matsumura, Tatsuya Kitagawa, Motoi Hashiba, Nobuto Okamoto, Hiroaki Takeuchi, Masafumi Ono, Toshiji Saibara
Organizer
The 24th United European Gastroenterology Week, Gastroenterology conference
Place of Presentation
Vienna
Year and Date
2016-10-15
Related Report
Int'l Joint Research
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