| Project/Area Number |
15K08655
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Yasuda Hiroshi 聖マリアンナ医科大学, 医学部, 教授 (80262129)
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| Co-Investigator(Kenkyū-buntansha) |
山本 博幸 聖マリアンナ医科大学, 医学部, 准教授 (40332910)
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| Research Collaborator |
Koshikawa Naohiko
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ラミニンγ２短鎖 / 肝細胞癌 / バイオマーカー / PIVKA-2 / AFP / サーベイランス / ラミニンγ2 / Ln-γ2 / 腫瘍マーカー |
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| Outline of Final Research Achievements |
Laminin (Ln)-332 consists of α3, β3, and γ2 chains, which mediate epithelial cell adhesion to the basement membrane. Ln-γ2 is frequently expressed as a monomer in the invasion front of several types of malignant tissues without simultaneous expression of Ln-α3 and/or Ln-β3 chains. Moreover, monomeric Ln-γ2 ( Ln-γ2m) induces tumor cell proliferation and migration in vitro. These unique biological activities indicate that Ln-γ2m could be a candidate biomarker for early cancer surveillance. We have developed monoclonal antibodies to specifically detect Ln-γ2m, and devised a highly sensitive method to measure serum Ln-γ2m levels using a fully automated CLIA. We evaluated its diagnostic value in sera from patients with several digestive cancers, including HCC, and found serum Ln-γ2m to be a clinically available biomarker for HCC surveillance. The combination of Ln-γ2m and PIVKA-2 may be more sensitive for clinical diagnosis of HCC than any currently used combination.
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| Academic Significance and Societal Importance of the Research Achievements |
５大癌の一つである肝癌は、今後ウイルス性肝炎を背景とするものは減少し、サーベイランスが困難な生活習慣病を背景とする非B非C型が増加すると予測される。難治癌である膵癌の罹患率は特に高齢者で増加しており、2013年の死亡数はほぼ肝癌と同数の3万人である。東アジアではウイルス肝炎罹患率が高く、欧米では非アルコール性脂肪肝に伴う肝細胞癌が増加している。肝癌・膵癌の制圧は世界的に重要な課題である。癌の新たなスクリーニング法の確立が急務である。
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