| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Sequential acquisition of mutations in drug-resistance associating genes causes an emergence of multidrug-resistant Mycobacterium tuberculosis (MDR-MTB). The Beijing genotype of MTB is known to be associating with MDR, and its prevalence among MDR-MTB population was significantly higher than that in non-MDR MTB in three TB high-burden countries; Myanmar, Nepal and Thailand. The majority of the Beijing type isolates belonged to the “modern” subtype and most of them had putative KatG S315T and RpsL K43R mutations suggesting those were isoniazid and streptomycin resistant. Those genetic characteristics can be used as a marker of transmissible MDR-MTB; thus, we have developed simple detection methods including a multiplex PCR to identify the “modern” type of the Beijing lineage and a line probe assay to diagnose isoniazid resistance.
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