• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of minimal-invasive and quantitative analytical method for nitrated DNA damage, and its application to risk assessment of inflammation-related carcinogenesis

Research Project

Project/Area Number 15K08787
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene and public health
Research InstitutionSuzuka University of Medical Science

Principal Investigator

Kawanishi Shosuke  鈴鹿医療科学大学, 薬学部, 教授 (10025637)

Co-Investigator(Kenkyū-buntansha) 大西 志保  鈴鹿医療科学大学, 薬学部, 助教 (80511914)
Co-Investigator(Renkei-kenkyūsha) MA Ning  鈴鹿医療科学大学, 医療科学研究科, 教授 (30263015)
TABATA Tsutomu  三重大学, 医学研究科, 准教授 (40252358)
IIJIMA Katsunori  秋田大学, 医学研究科, 教授 (60375003)
Research Collaborator MURATA Mariko  三重大学大学院, 医学研究科, 教授 (10171141)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsがん予防 / 炎症 / DNA損傷
Outline of Final Research Achievements

Chronic infection and inflammation have been recognized as important factors for carcinogenesis. Inflammation is estimated to contribute to approximately 25 % of human cancers. It is urgent problem to be solved, the risk assessment in early phase and prevention of inflammation-related carcinogenesis. We have reported that 8-nitroguanine, nitrative DNA damage specifically generated under inflammation, is generated in cancerous site before carcinogenesis. Therefore, 8-nitroguanine is hopeful marker for risk assessment of inflammation-related carcinogenesis. However, it is difficult to analyze 8-nitroguanine with quantitativity and minimal-invasiveness. In this study, we successed to confirm the amount of 8-nitroguanine quantitatively with using high performance liquid chromatography coupled with an electrochemical detector (HPLC-ECD).

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015

All Journal Article (13 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 13 results,  Open Access: 8 results,  Acknowledgement Compliant: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Nitrative Stress and Tau Accumulation in Amyotrophic Lateral Sclerosis/Parkinsonism-Dementia Complex (ALS/PDC) in the Kii Peninsula, Japan2018

    • Author(s)
      Hata Yukiko、Ma Ning、Yoneda Misao、Morimoto Satoru、Okano Hideyuki、Murayama Shigeo、Kawanishi Shosuke、Kuzuhara Shigeki、Kokubo Yasumasa
    • Journal Title

      Frontiers in Neuroscience

      Volume: 11 Pages: 751-751

    • DOI

      10.3389/fnins.2017.00751

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrative DNA damage in cultured macrophages exposed to indium oxide2018

    • Author(s)
      Afroz T, Hiraku Y, Ma N, Ahmed S, Oikawa S, Kawanishi S, Murata M
    • Journal Title

      Journal of Occupational Health

      Volume: 60 Issue: 2 Pages: 148-155

    • DOI

      10.1539/joh.17-0146-OA

    • NAID

      130006565471

    • ISSN
      1341-9145, 1348-9585
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mechanism of oxidative DNA damage induced by metabolites of carcinogenic naphthalene2018

    • Author(s)
      Ohnishi Shiho、Hiraku Yusuke、Hasegawa Keishi、Hirakawa Kazutaka、Oikawa Shinji、Murata Mariko、Kawanishi Shosuke
    • Journal Title

      Mutat Res.

      Volume: 827 Pages: 42-49

    • DOI

      10.1016/j.mrgentox.2018.01.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiling of hepatocarcinogenesis in a mouse model of chronic hepatitis B2017

    • Author(s)
      Nosaka Takuto、Naito Tatsushi、Hiramatsu Katsushi、Ohtani Masahiro、Nemoto Tomoyuki、Marusawa Hiroyuki、Ma Ning、Hiraku Yusuke、Kawanishi Shosuke、Yamashita Taro、Kaneko Shuichi、Nakamoto Yasunari
    • Journal Title

      PLoS One.

      Volume: 12 Issue: 10 Pages: e0185442-e0185442

    • DOI

      10.1371/journal.pone.0185442

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crosstalk between DNA Damage and Inflammation in the Multiple Steps of Carcinogenesis2017

    • Author(s)
      Kawanishi Shosuke、Ohnishi Shiho、Ma Ning、Hiraku Yusuke、Murata Mariko
    • Journal Title

      Int J Mol Sci.

      Volume: 18 Issue: 8 Pages: 1808-1808

    • DOI

      10.3390/ijms18081808

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nitrative DNA damage induced by carbon-black nanoparticles in macrophages and lung epithelial cells2017

    • Author(s)
      Hiraku Y, Nishikawa Y, Ma N, Afroz T, Mizobuchi K, Ishiyama R, Matsunaga Y, Ichinose T, Kawanishi S, Murata S.
    • Journal Title

      Mutat Res Gen Tox En.

      Volume: 818 Pages: 7-16

    • DOI

      10.1016/j.mrgentox.2017.04.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrative and oxidative DNA damage in infection-related carcinogenesis in relation to cancer stem cells.2017

    • Author(s)
      Kawanishi S, Ohnishi S, Ma N, Hiraku Y, Oikawa S, Murata M.
    • Journal Title

      Genes Environ

      Volume: 38 Issue: 1 Pages: 26-26

    • DOI

      10.1186/s41021-016-0070-8

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inflammation-related DNA damage and cancer stem cell markers in nasopharyngeal carcinoma.2016

    • Author(s)
      Wang S, Ma N, Zhao W, Midorikawa K, Kawanishi S, Hiraku Y, Oikawa S, Zhang Z, Huang GW, Murata M.
    • Journal Title

      Mediators Inflamm.

      Volume: 2016 Pages: 9343460-9343460

    • DOI

      10.1155/2016/9343460

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The enhancement of oxidative DNA damage by anti-diabetic metformin, buformin, and phenformin, via nitrogen-centered radicals.2016

    • Author(s)
      Ohnishi S, Mizutani H, Kawanishi S.
    • Journal Title

      Free Radic Res.

      Volume: 50 Issue: 8 Pages: 929-937

    • DOI

      10.1080/10715762.2016.1204651

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA Damage in CD133-Positive Cells in Barrett's Esophagus and Esophageal Adenocarcinoma.2016

    • Author(s)
      Thanan R, Ma N, Hiraku Y, Iijima K, Koike T, Shimosegawa T, Murata M, Kawanishi S.
    • Journal Title

      Mediators Inflamm.

      Volume: 2016 Pages: 7937814-7937814

    • DOI

      10.1155/2016/7937814

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation.2016

    • Author(s)
      Hiraku Y, Guo F, Ma N, Yamada T, Wang S, Kawanishi S, Murata M.
    • Journal Title

      Part Fibre Toxicol.

      Volume: 13 Issue: 1 Pages: 16-16

    • DOI

      10.1186/s12989-016-0127-7

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neuronal differentiation of human iPS cells induced by baicalin via regulation of bHLH gene expression.2015

    • Author(s)
      Morita A, Soga K, Nakayama H, Ishida T, Kawanishi S, Sato EF.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 465 Issue: 3 Pages: 458-463

    • DOI

      10.1016/j.bbrc.2015.08.039

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Oxidative DNA damage induced by metabolites of chloramphenicol, an antibiotic drug.2015

    • Author(s)
      Ohnishi S, Murata M, Ida N, Oikawa S, Kawanishi S.
    • Journal Title

      Free Radic Res.

      Volume: 49 Issue: 9 Pages: 1165-1172

    • DOI

      10.3109/10715762.2015.1050963

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 寄生虫感染による膀胱癌患者組織における炎症関連DNA損傷とCOX2発現2017

    • Author(s)
      大西志保、馬寧、村田真理子、平工雄介、及川伸二、小林果、川西正祐
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      フェニックスシーガイアリゾート シーガイアコンベンションセンター(宮崎市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物ポリフェノール化合物シリビニンのDNA損傷性と活性酸素を介したアポトーシス2017

    • Author(s)
      水谷秀樹、大野奈々、松浦史佳、平工雄介、川西正祐
    • Organizer
      日本酸化ストレス学会東海支部第 5 回学術集会
    • Place of Presentation
      愛知学院大学名城公園キャンパス(名古屋市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 発がん性ナフタレン代謝物による酸化的DNA損傷機構2016

    • Author(s)
      大西志保、平工雄介、平川和貴、及川伸二、村田真理子、川西正祐
    • Organizer
      日本環境変異原学会第45回大会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ビグアニド系糖尿病治療薬による酸化的DNA損傷の増強効果2016

    • Author(s)
      大西志保、水谷秀樹、川西正祐
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Nitrative DNA damage induced by multi-walled carbon nanotube in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation.2016

    • Author(s)
      Yusuke Hiraku, Feiye Guo, Ning Ma, Tatsuhiko Yamada, Shumin Wang, Shosuke Kawanishi, Mariko Murata
    • Organizer
      11th International Particle Toxicology Conference
    • Place of Presentation
      Singapore, Biopolis Matrix
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] インジウム化合物に曝露した培養細胞におけるニトロ化DNA損傷2016

    • Author(s)
      平工雄介、Tahmina Afroz、馬寧、川西正祐、村田真理子
    • Organizer
      日本産業衛生学会東海地方会
    • Place of Presentation
      浜松医科大学(浜松市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 多層カーボンナノチューブで処理した肺上皮細胞におけるニトロ化DNA損傷:Toll様受容体9の役割2015

    • Author(s)
      平工雄介、馬寧、王淑民、川西正祐、村田真理子
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] Inflammation-related DNA damage in relation to the expression of cancer stemness markers in human nasopharyngeal carcinoma.2015

    • Author(s)
      Wang S, Ma N, Kawanishi S, Zhao W, Midorikawa K, Hiraku Y, Oikawa S, Zhang Z, Huang G, Murata M
    • Organizer
      SFRR-E/SNFS Meeting Stuttgart 2015: Redox Biology Meets Nutrition
    • Place of Presentation
      University of Hohenheim (Stuttgart, Germany)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi