Development of a new detection system of autoantibodies in Hashimoto encephalopathy and search for the pleiotropy
| Project/Area Number |
15K09440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
YONEDA Makoto 福井県立大学, 看護福祉学部, 教授 (70270551)
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| Co-Investigator(Kenkyū-buntansha) |
篁 俊成 金沢大学, 医学系, 教授 (00324111)
樋口 理 独立行政法人国立病院機構長崎川棚医療センター(臨床研究部), その他の研究科, 研究員(移行) (50361720)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 橋本脳症 / 抗NAE抗体 / 分子診断マーカー / LIPS法 / 生物発光 |
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| Outline of Final Research Achievements |
Hashimoto’s encephalopathy is an autoimmune neuropsychiatric disorder associated with chronic thyroiditis, Hashimoto’s thyroiditis. We discovered specific autoantibodies (Abs) against NAE protein in the patient’s serum. In this research, we developed a new detection system of anti-NAE Abs by a bioluminescence/immunoprecipitation method (luciferase immunoprecipitation systems; LIPS). We were succeeded in the construction of a plasmid expressing a NAE/luciferase chimeric protein, expressed it in a human cultured cells HEK293, and purified the recombinant protein. We verified the immunoreactivity and bioluminescence of the recombinant NAE/luciferase chimeric protein with the patient’s serum. This LIPS system to detect anti-NAE Abs showed a similar tendency to that in the previous immunoblot method.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Case of Hashimoto's encephalopathy showing atypical clinical course with magnetic resonance imaging abnormalities2017
Author(s)
Hirose D, Hirao K, Kaneko Y, Fukasawa R, Sato T, Shimizu S, Kanetaka H, Umahara T, Sakurai H, Yoneda M, Hanyu H
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Journal Title
Geriatr Gerontol Int
Volume: 17(8)
Issue: 8
Pages: 1235-1237
DOI
Related Report
Peer Reviewed
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[Journal Article] Prevalence of Autoantibodies and the Efficacy of Immunotherapy for Autoimmune Cerebellar Ataxia2016
Author(s)
Nanri K, Okuma M, Sato S, Yoneda M, Taguchi T, Mitoma H, Yamada J, Unezaki S, Nagatani T, Otsubo S, Sugawara M, Tanaka N, Mizusawa H
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Journal Title
Internal Medicine
Volume: 55
Issue: 5
Pages: 449-454
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed / Open Access
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[Journal Article] Consensus Paper: NeuroimmuneMechanisms of Cerebellar Ataxias2015
Author(s)
Mitoma H, Adhikari K, Aeschlimann D, Chattopadhyay P, Hadjivassiliou M, Hampe CS, Honnorat J, Joubert B, Kakei S, Lee J, Manto M, Matsunaga A, Mizusawa H,Nanri K, Shanmugarajah P, Yoneda M, Yuki N.
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Journal Title
Cerebellum
Volume: on line
Issue: 2
Pages: 213-32
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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