Investigation for iron sensing mechanism via the direct regulation of hepcidin expression by serum ferritin protein
Project/Area Number |
15K09446
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Asahikawa Medical College |
Principal Investigator |
IKUTA KATSUYA 旭川医科大学, 医学部, 講師 (00396376)
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Co-Investigator(Kenkyū-buntansha) |
進藤 基博 旭川医科大学, 医学部, 講師 (10396377)
伊藤 巧 旭川医科大学, 大学病院, 特任助教 (80548686)
土岐 康通 旭川医科大学, 大学病院, 医員 (90596280)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 鉄代謝 / フェリチン / ヘプシジン / 鉄感知機構 / 血清フェリチン |
Outline of Final Research Achievements |
The present study was performed to determine the possibility that serum ferritin protein which increases in iron overload directly regulate iron regulatory peptide hepcidin produced from hepatocytes. Upregulation of HAMP (hepcidin) gene in human hepatoma-derived HepG2 cells was observed when cells were incubated with human spleen-derived ferritin protein. Because ferritin protein is present as heteropolymer of L- (FTL) and H- (FTH) subunit of ferritin, the influences of each subunit were then determined. The medium incubated with FTL- or FTH-overexpressed HEK293 cells, and FTL or FTH protein purified from transformed E. Coli were used; however, HAMP expression was decreased contrary. The results obtained from this study seemed to be discrepant, but there is a possibility that ferritin might manipulate HAMP expression quite finely utilizing bidirectional effect. This study will be further continued considering iron contents in ferritin, and the ratio of each subunit in heteropolymer.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Reticulocyte hemoglobin equivalent as a potential marker for diagnosis of iron deficiency.2017
Author(s)
Toki Y, Ikuta K, Kawahara Y, Niizeki N, Kon K, Enomoto M, Tada Y, Hatayama M, Yamamoto M, Ito S, Shindo M, Kikuchi Y, Inoue M, Sato K, Fujiya M, Okumura T.
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Journal Title
International Journal of Hematology
Volume: 印刷中
Pages: 印刷中
DOI
Related Report
Peer Reviewed
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[Journal Article] Iron overload patients with unknown etiology from national survey in Japan.2017
Author(s)
Ikuta K, Hatayama M, Addo L, Toki Y, Sasaki K, Tatsumi Y, Hattori A, Kato A, Kato K, Hayashi H, Suzuki T, Kobune M, Tsutsui M, Gotoh A, Aota Y, Matsuura M, Hamada Y, Tokuda T, Komatsu N, Kohgo Y.
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Journal Title
International Journal of Hematology
Volume: 105
Pages: 353-360
DOI
Related Report
Peer Reviewed
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[Journal Article] Iron-induced epigenetic abnormalities of mouse bone marrow through aberrant activation of aconitase and isocitrate dehydrogenase.2016
Author(s)
Yamamoto M, Tanaka H, Toki Y, Hatayama M, Ito S, Addo L, Shindo M, Sasaki K, Ikuta K, Ohtake T, Fujiya M, Torimoto Y, Kohgo Y.
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Journal Title
International Journal of Hematology
Volume: 104
Pages: 491-501
DOI
Related Report
Peer Reviewed
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[Journal Article] A selective splicing variant of hepcidin mRNA in hepatocellularcarcinoma cell lines.2016
Author(s)
Toki Y, Sasaki K, Tanaka H, Yamamoto M, Hatayama M, Ito S, Ikuta K, Shindo M, Hasebe T, Nakajima S, Sawada K, Fujiya M, Torimoto Y, Ohtake T, Kohgo Y.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 476
Pages: 501-507
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vivo behavior of NTBI revealed by automated quantification system.2016
Author(s)
Ito S, Ikuta K, Kato D, Lynda A, Shibusa K, Niizeki N, Toki Y, Hatayama M, Yamamoto M, Shindo M, Iizuka N, Kohgo Y, Fujiya M.
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Journal Title
International Journal of Hematology
Volume: 104
Pages: 175-181
DOI
Related Report
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[Journal Article] The three isoforms of hepcidin in human serum and their processing determined by liquid chromatography-tandem mass spectrometry (LC-tandem MS).2016
Author(s)
Addo L, Ikuta K, Tanaka H, Toki Y, Hatayama M, Yamamoto M, Ito S, Shindo M, Sasaki Y, Shimonaka Y, Fujiya M, Kohgo Y.
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Journal Title
International Journal of Hematology
Volume: 103
Pages: 34-43
Related Report
Peer Reviewed
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[Presentation] Update of the hereditary hemochromatosis-related gene mutation analysis in Japan.2017
Author(s)
Yasumichi Toki, Katsuya Ikuta, Masatomo Ishioh, Takeshi Saito, Mayumi Hatayama, Masayo Yamamoto, Motohiro Shindo, Yoshihiro Torimoto, Yasuaki Tatsumi, Ayako Kato, Hisao Hayashi, Koichi Kato, Toshikatsu Okumura.
Organizer
第79回日本血液学会学術集会
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[Presentation] Reticulocyte hemoglobin equivalent (RET-He) is a possible marker for diagnosis of iron deficiency.2015
Author(s)
Yasumichi Toki, Katsuya Ikuta, Satoshi Ito, Mayumi Hatayama, Masayo Yamamoto, Motohiro Shindo, Yoshihiro Torimoto, Kazuya Sato, Junki Inamura, Takaaki Hosoki, Kazuhiko Ichiki, Mikihiro Fujiya.
Organizer
第77回日本血液学会学術集会
Place of Presentation
石川県立音楽堂(金沢)
Year and Date
2015-10-16 – 2015-10-16
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