Establishment of pediatric solid tumor development model using human embryonic stem cell differentiation induction system

• Project/Area Number: 15K09651
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto University
• Principal Investigator: Umeda Katsutsugu 京都大学, 医学研究科, 助教 (80397538)
• Research Collaborator: NODOMI Seishiro 京都大学, 医学研究科, 医員 ; KINEHARA Takako 京都大学, 医学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ES細胞 / 小児固形腫瘍 / 神経堤細胞

Outline of Final Research Achievements

CD271high neural crest cells developed during six-day differentiation induction of undifferentiated ES cells under a serum-free condition with Nodal/activin. FCM analysis of surface markers expressed during the neural crest differentiation revealed that various cell lines of pediatric solid tumors expressed CD146. Treatment with a polyclonal antibody against CD146 effectively suppressed in vivo tumor growth of malignant rhabdoid tumor and neuroblastoma cells in immunodeficient mice.