identification for leukemic stem cells using microRNA sensor vector for developing new therapy

• Project/Area Number: 15K09652
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto University
• Principal Investigator: Hiramatsu Hidefumi 京都大学, 医学研究科, 講師 (40362503)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: microRNA / 白血病 / 小児急性白血病 / 急性リンパ性白血病 / 白血病幹細胞

Outline of Final Research Achievements

Recently microRNAs are investigated as a therapeutic marker or a target for hematologic malignancies. Based on the results of miR analysis of primary pediatric acute lymphoblastic leukemia (ALL), we picked up several target miRs including miR-146a and investigated its activities in various ALL cell lines using a miR sensing vector and a miR knock down vector. Mir-146a is generally upregulated and differentially expressed in most cell lines. Knocking down of this miR resulted in 30-50% reduction of colony formation. Underlying molecular mechanism is under investigation by RNA-seq.

Research Products

• [Int'l Joint Research] San Raffaele Hospital(イタリア)
• [Journal Article] Influence of post-transplant mucosal-associated invariant T cell recovery on the development of acute graft-versus-host disease in allogeneic bone marrow transplantation (2018)
  • Author(s): Kawaguchi Koji、Umeda Katsutsugu、Hiejima Eitaro、Iwai Atsushi、Mikami Masamitsu、Nodomi Seishiro、Saida Satoshi、Kato Itaru、Hiramatsu Hidefumi、Yasumi Takahiro、Nishikomori Ryuta、Kondo Tadakazu、Takaori-Kondo Akifumi、Heike Toshio、Adachi Souichi
  • Journal Title: International Journal of Hematology Volume: 印刷中 Issue: 1 Pages: 66-75
  • DOI: 10.1007/s12185-018-2442-2
  • Peer Reviewed
• [Journal Article] Impact of post-transplant minimal residual disease on the clinical outcome of pediatric acute leukemia (2017)
  • Author(s): Umeda Katsutsugu、Iwai Atsushi、Kawaguchi Koji、Mikami Masamitsu、Nodomi Seishiro、Saida Satoshi、Hiramatsu Hidefumi、Heike Toshio、Ohmori Katsuyuki、Adachi Souichi
  • Journal Title: Pediatric Transplantation Volume: 21 Issue: 4
  • DOI: 10.1111/petr.12926
  • Peer Reviewed