Development of molecular marker and novel therapy for FAP

• Project/Area Number: 15K10154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: Yamano Tomoki 兵庫医科大学, 医学部, 准教授 (00599318)
• Co-Investigator(Kenkyū-buntansha): 冨田 尚裕 兵庫医科大学, 医学部, 教授 (00252643) ; 久保 秀司 兵庫医科大学, 医学部, 准教授 (10441320)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 家族性大腸腺腫症 / マイクロRNA / バイオマーカー / デスモイド

Outline of Final Research Achievements

We looked for biomarker of FAP in plasma miRNA to detect existence of FAP and associated comorbidities. We compared plasma from five FAP patients before total proctocolectomy and three healthy volunteers using next generation sequence. Candidates included mir143-5p (7.6 times increase), mir143-3p (6.2 times increase), mir96-5p (10.9 times increase), mir183-5p (2.9 times increase), mir885-5p (one-eighth decrease). Among these candidates, we validated mir-143 because mir143-3p demonstrated high copy number in plasma. Decrease of mir143 in polyp of FAP patients was also reported. We measure relative expression of mir143 in eight FAP patients included three patients assessed by NGS. Relative expression of mir143 was 1.48, 1.28, 1.9, 1.46 and 0.76 in FAP patients before surgery. Relative expression of mir143 was 0.42, 0.44 and 0.2 in FAP patients after surgery. These data indicated mir143 expression might decrease after surgery, which meant polyposis influenced mir143 expression.

Research Products

• [Journal Article] Management strategies in Lynch syndrome and familial adenomatous polyposis: a national healthcare survey in Japan. (2017)
  • Author(s): Yamano T, Hamanaka M, Babaya A, Kimura K, Kobayashi M, Fukumoto M, Tsukamoto K, Noda M, Matsubara N, Tomita N, Sugihara K.
  • Journal Title: Cancer Science Volume: 108 Issue: 2 Pages: 243-249
  • DOI: 10.1111/cas.13123
  • Peer Reviewed / Open Access
• [Journal Article] The usefulness of Mohs’ paste for the treatment of a malignant wound caused by a giant intra-abdominal desmoid tumor (2015)
  • Author(s): Tomoki Yamano • Masayoshi Kobayashi • Mie Yoshimura • Kiyoshi Tsukamoto • Masafumi Noda • Nagahide Matsubara • Akira Nagita • Naohiro Tomita
  • Journal Title: International Cancer Conference Journal Volume: 4 Issue: 4 Pages: 258-261
  • DOI: 10.1007/s13691-015-0214-x
  • Peer Reviewed
• [Presentation] FAP患者における血漿中miRNAのバイオマーカーとしての有効性の検討 (2017)
  • Author(s): 山野智基、木村慶、馬場谷彰仁、濵中美千子、小林政義、塚本 潔、 野田雅史、今田絢子、宋 智亨、池田正孝、冨田尚裕
  • Organizer: 第87回大腸癌研究会
• [Presentation] 当院におけるFAP術後に発生したデスモイド腫瘍症例の検討 (2015)
  • Author(s): 小林正義、吉村美衣、別府直仁、塚本潔、山野智基、野田雅史、松原長秀、冨田尚裕
  • Organizer: 第70回 日本消化器外科学会
  • Place of Presentation: アクトシティ―浜松(静岡県・浜松市）
  • Year and Date: 2015-07-15
• [Presentation] 当科における家族性大腸腺腫症(FAP)に合併したデスモイド腫瘍に対するドキソルビシン/ダカルバジン併用化学療法の検討 (2015)
  • Author(s): 浜中美千子、山野智基、小林政義、吉村美衣、別府直仁、塚本潔、野田雅史、松原長秀、田村和朗、冨田尚裕
  • Organizer: 第21回 日本家族性腫瘍学会学術集会
  • Place of Presentation: ラフレさいたま（埼玉県・さいたま市）
  • Year and Date: 2015-06-05