• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of human iPS cell-derived multidisciplinary thick viable organized tissues aiming for myocardial regenerative therapy

Research Project

Project/Area Number 15K10210
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cardiovascular surgery
Research InstitutionKyoto University

Principal Investigator

Nakata Tomohiro  京都大学, 医学研究科, 助教 (00559685)

Co-Investigator(Kenkyū-buntansha) 池田 義  京都大学, 医学研究科, 准教授 (40281092)
田畑 泰彦  京都大学, ウイルス・再生医科学研究所, 教授 (50211371)
Co-Investigator(Renkei-kenkyūsha) YAMASHITA JUN  京都大学, その他部局等, 教授 (50335288)
Research Collaborator MASUMOTO HIDETOSHI  京都大学, 大学院医学研究科, 特定助教 (70645754)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsiPS細胞 / バイオエンジニアリング / 心臓再生 / 心筋疾患外科学 / 拡張型心筋症 / バイトエンジニアリング
Outline of Final Research Achievements

We established a technology to generate human iPS cell-derived thick viable cardiac tissues. We validated the therapeutic potential of the tissues for porcine sub-acute myocardial infarction model, rat chronic myocardial infarction model and hamster dilated cardiomyopathy modei. Histological studies revealed the therapeutic mechanisms including the attenuation of fibrosis, angiogenesis, the attenuation of apoptosis and cardiomyocyte hypertrophy. We also established the method to generate cardiac tissue sheets and thick viable tissues from clinical-graded stock human iPS cells from Center for iPS cell Research and Application, Kyoto University. There results including the validation of the therapeutic effects of the bioengineeded tissue and the therapeutic mechanism would serve as a basis for iPS cell-based cardiac regenerative therapy.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (21 results) (of which Int'l Joint Research: 15 results) Remarks (1 results)

  • [Journal Article] Impact of Cell Composition and Geometry on Human Induced Pluripotent Stem Cells-Derived Engineered Cardiac Tissue.2017

    • Author(s)
      Nakane T, Masumoto H, Tinney JP, Yuan F, Kowalski WJ, Ye F, LeBlanc AJ, Sakata R, Yamashita JK, Keller BB.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 45641-45641

    • DOI

      10.1038/srep45641

    • NAID

      120006309290

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fetal mesenchymal stem cells ameliorate acute lung injury in a rat cardiopulmonary bypass model.2017

    • Author(s)
      7.Taki T, Masumoto H, Funamoto M, Minakata K, Yamazaki K, Ikeda T, Sakata R.
    • Journal Title

      The Journal of Thoracic and Cardiovascular Surgery

      Volume: 153 Issue: 3 Pages: 726-734

    • DOI

      10.1016/j.jtcvs.2016.10.014

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Prevention of abdominal aortic aneurysm progression by oral administration of green tea polyphenol in a rat model.2016

    • Author(s)
      Setozaki S, Minakata K, Masumoto H, Hirao S, Yamazaki K, Kuwahara K, Ikeda T, Sakata R.
    • Journal Title

      J Thorac Cardiovasc Surg.

      Volume: - Issue: 6 Pages: 1803-1812

    • DOI

      10.1016/j.jvs.2016.06.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Green Tea Polyphenol Prevents Diabetic Rats From Acute Kidney Injury After Cardiopulmonary Bypass.2016

    • Author(s)
      Funamoto M, Masumoto H, Takaori K, Taki T, Setozaki S, Yamazaki K, Minakata K, Ikeda T, Hyon SH, Sakata R.
    • Journal Title

      Ann Thorac Surg

      Volume: 101 Issue: 4 Pages: 1507-13

    • DOI

      10.1016/j.athoracsur.2015.09.080

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.2015

    • Author(s)
      Matsuo T, Masumoto H, Tajima S, Ikuno T, Katayama S, Minakata K, Ikeda T, Yamamizu K, Tabata Y, Sakata R, Yamashita JK.
    • Journal Title

      Scientific Reports

      Volume: 5:16842 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep16842

    • NAID

      120005753829

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Mesh-shaped engineered cardiac tissue generated from human iPS cell for cardiac regeneration2017

    • Author(s)
      Nakane T, Masumoto H, Minakata K, Ikeda T, Minatoya K, Yamashita J, Sakata R
    • Organizer
      第81回日本循環器学会学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transplantation of human induced pluripotent stem cell-derived cardiac tissue ameliorates heart failure in delated cardiomyopathy of hamster model2017

    • Author(s)
      瀧本 真也,升本 英利,南方 謙二,池田 義,湊谷 謙司,坂田 隆造,山下 潤
    • Organizer
      第81回日本循環器学会学術集会
    • Place of Presentation
      金沢市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Spring-8における位相差CTによる拡張型心筋症ハムスターに対する立体的組織評価の確立2017

    • Author(s)
      平尾 慎吾,南方 謙司,升本 英利,瀧本 真也,坂本 和久,中津 太郎,中田 朋宏,山﨑 和裕,池田 義,湊谷 謙司,坂田 隆造
    • Organizer
      第47回日本心臓血管外科学会学術総会
    • Place of Presentation
      東京都
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 慢性期ラット心筋梗塞に対するbFGF徐放後ヒトiPS由来心筋組織シート移植の治療効果2017

    • Author(s)
      李 子澎,升本 英利,南方 謙二,川東 正英,瀧本 真也,平尾 慎吾,池田 義,坂田 隆造,湊谷 謙司
    • Organizer
      第47回日本心臓血管外科学会学術総会
    • Place of Presentation
      東京都
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 拡張型心筋症ハムスターに対するヒトiPS細胞由来心臓組織シート移植の有効性2017

    • Author(s)
      瀧本 真也,升本 英利,南方 謙二,池田 義,湊谷 謙司
    • Organizer
      第47回日本心臓血管外科学会学術総会
    • Place of Presentation
      東京都
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞由来心筋・血管系細胞を用いた移植用網目状心臓組織パッチの開発2017

    • Author(s)
      中根 武一郎,升本 英利,南方 謙二,池田 義、湊谷 謙司
    • Organizer
      第47回日本心臓血管外科学会学術総会
    • Place of Presentation
      東京都
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Three-dimensional visualization and morphometry of cardidac tissue in a hamster DCM model by synchrotron radiation-based x-ray phasecontrast imaging at SPring-82017

    • Author(s)
      Hirao S, Minakata K, Masumoto H, Takimoto S, Hoshino M, Yagi N, Shirai M, Minatoya K, Sakata R
    • Organizer
      第81回日本循環器学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Three-dimenshional visualization and morphometry of caediac tiddue in a hamster DCM model by synchrotron radiation-based X-ray phase-contrast imaging at Spring-82017

    • Author(s)
      Hirao S, Masumoto H, Hoshino M, Minakata K, Takimoto S, Ikeda T, Yamashita J.K, Yagi N, Minatoya K.
    • Organizer
      European Society of Cardiology Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-dimensional Visualization and Morphometry of Cardiac Tissue in a Hamster DCM Model by Synchrotron Radiation-based X-ray Phase-contrast Imaging at Spring-82017

    • Author(s)
      Hirao S, Masumoto H, Hoshino M, Kanemitsu H, Ueyama K, Yamazaki K, Ikeda T, Yamashita J.K, Yagi N, Minatoya K
    • Organizer
      American Heart Association 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The therapeutic potential of human iPS cell-derived cardiac tissue onto a porcine myocardial infarction model.2017

    • Author(s)
      Hirao S, Masumoto H, Nishio H, Kawatou M, Li Z, Kanemtitsu H, Ueyama K,Yamazaki K,Ikeda T,Tabata Y, Yamashita J.K, Minatoya K.
    • Organizer
      American Heart Association 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞由来心筋・血管系細胞を用いた移植用網目状心臓組織パッチの開発2017

    • Author(s)
      中根 武一郎,升本 英利,池田 義,湊谷 謙司,山下 潤,Bradley B.Keller
    • Organizer
      第16回日本心臓血管外科再生治療研究会
    • Place of Presentation
      東京都
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Impact of geometry and cell density on structural and functional maturation in large-format mesh engineered cardiac tissues2016

    • Author(s)
      Nakane T, Masumoto H, J P. Tinney, F Yuan, W J. Kowalski, F Ye, Minakata K, Ikeda T, Sakata R, Yamashita J.K, B B.Keller
    • Organizer
      AHA American Heart Association
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Sequential treatment with sustained-release of basic fibroblast growth factor followed by transplantation of human induced pluripotent stem cell-derived cardiac tissue improved left ventricular function in a rat chronic myocardial infarction model2016

    • Author(s)
      Zipeng Li, Masumoto H, Minakata K, Kawatou M, Takimoto S, Hirao S, Ikeda T, Tabata Y, Yamashita J.K, Sakata R
    • Organizer
      AHA American Heart Association
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A technology for cardiac regeneration using human iPS cell-derived cardiac tissue including multiple cardiovascular lineages2016

    • Author(s)
      Masumoto H, Yamamizu K, Ikuno T, Takakubo H, Minakata K, Ikeda T, Tabata Y, Yamashita J.K.
    • Organizer
      AHA American Heart Association
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The efficacy of transplantation of human iPS cell derived cardiac tissue for dilated cardiomyopathy hamster model2016

    • Author(s)
      Takimoto S, Masumoto H, Minakata K, Tabata Y, Yamashita J.K.
    • Organizer
      AHA American Heart Association
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞由来心筋細胞および血管構成細胞を含む三次元人工心筋組織による心筋再生についての検討2016

    • Author(s)
      升本 英利,中根 武一郎,南方 謙二,池田 義,坂田 隆造
    • Organizer
      第69回日本胸部外科学会定期学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 拡張型心筋症ハムスターに対するヒトiPS細胞由来心臓組織シート移植の有効性2016

    • Author(s)
      瀧本 真也,升本 英利,南方 謙二,池田 義,坂田 隆造
    • Organizer
      第69回日本胸部外科学会定期学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトiPS細胞由来心筋・血管系細胞を用いた移植用大型心臓パッチの開発2016

    • Author(s)
      中根 武一郎,升本 英利,南方 謙二,池田 義,坂田 隆造
    • Organizer
      第69回日本胸部外科学会定期学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Combined transplantation of human iPS cell-derived cardiac tissue sheets and sustained-release of bFGF improved left ventricular function in a rat chronic myocardial infarction model2016

    • Author(s)
      李 子澎,升本 英利,南方 謙二,川東 正英,瀧本 真也,平間 大介,平尾 慎吾,池田 義,坂田 隆造
    • Organizer
      第69回日本胸部外科学会定期学術集会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 心臓血管領域における再生治療の展望2016

    • Author(s)
      南方 謙二,升本 英利,池田 義
    • Organizer
      第37回日本炎症・再生医学会
    • Place of Presentation
      京都市
    • Year and Date
      2016-06-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Cardiac tissue sheet including human iPS cell-derived cardiomyocytes and vascular cells restores impaired left ventricular systolic function of infarcted porcine hearts: a preclinical study.2015

    • Author(s)
      Masumoto H, Ishigami M, Aoki T, Takai F, Ikuno T, Kawatoh M, Minakata K, Ikeda T, Sakata R, Yamashita JK.
    • Organizer
      European Society of Cardiology (ESC) Congress
    • Place of Presentation
      London, UK
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 京都大学 心臓血管外科

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi