The role of the cleaved osteopintin in stone formation

• Project/Area Number: 15K10628
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Hamamoto Shuzo 名古屋市立大学, 大学院医学研究科, 講師 (80551267)
• Co-Investigator(Kenkyū-buntansha): 郡 健二郎 名古屋市立大学, その他部局等, 学長 (30122047) ; 戸澤 啓一 名古屋市立大学, 大学院医学研究科, 教授 (40264733) ; 安井 孝周 名古屋市立大学, 大学院医学研究科, 教授 (40326153) ; 岡田 淳志 名古屋市立大学, 大学院医学研究科, 講師 (70444966)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 尿路結石 / オステオポンチン / 細胞障害 / 腎結石 / 抗体 / バイオマーカー

Outline of Final Research Achievements

Osteopontin (OPN) plays a crucial role in the formation of renal calcium crystals, which are primarily induced by renal tubular cell injury. We have previously shown that the impaired RGD sequence,which serves as an adhesion motif that promotes cell attachment, inhibits renal crystal formation by using OPN-transgenic mice and OPN-knockout mice. In this study, thrombin-cleaved osteopontin plays a nonredundant role in renal crystal formation, which is a complex multistep process. A specific anti-OPN antibody contributes to the remarkable inhibition of early stage of renal crystal formation by suppressing RTC injury, the attachment of crystals to renal epithelium cells or crystal growth.

Research Products

• [Journal Article] Genome-wide gene expression profiling of Randall's plaques in calcium oxalate stone formers (2017)
  • Author(s): Taguchi K, Hamamoto S, Okada A, Unno R, Kamisawa H, Naiki T, Ando R, Mizuno K, Kawai N, Tozawa K, Kohri K, Yasui T
  • Journal Title: J Am Soc Nephrol Volume: 28 Issue: 1 Pages: 333-347
  • DOI: 10.1681/asn.2015111271
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A case of delayed radiation myelopathy of the thoracic vertebrae following low dose radiation therapy for metastatic renal cell carcinoma. (2017)
  • Author(s): Nozaki Satoshi, Naiki Taku, Hamamoto Shuzo, Ando Ryosuke, Iida Keitaro, Kawai Noriyasu, Mizuno Kentaro, Okada Atsushi, Umemoto Yukihiro, Yasui Takahiro
  • Journal Title: Urology Case Reports Volume: 11 Pages: 66-68
  • DOI: 10.1016/j.eucr.2017.01.005
• [Journal Article] Potassium-sodium citrate prevents the development of renal microcalculi into symptomatic stones in calcium stone-forming patients. (2017)
  • Author(s): Unno Rei, Taguchi Kazumi, Okada Atsushi, Ando Ryosuke, Hamamoto Shuzo, Kubota Yasue, Zuo Li, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 24 Issue: 1 Pages: 75-81
  • DOI: 10.1111/iju.13242
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ureteral wall thickness as a preoperative indicator of impacted stones in patients with ureteral stones undergoing ureteroscopic lithotripsy. (2017)
  • Author(s): Yoshida Takashi, Inoue Takaaki, Omura Naoto, Okada Shinsuke, Hamamoto Shuzo, Kinoshita Hidefumi, Matsuda Tadashi
  • Journal Title: Urology Volume: 106 Pages: 45-49
  • DOI: 10.1016/j.urology.2017.04.047
• [Journal Article] Two-year-old girl with impacted ureteral stone successfully treated with a single session of combined percutaneous nephrostomy and ureteroscopy. (2017)
  • Author(s): Sugino Teruaki, Hamamoto Shuzo, Unno Rei, Moritoki Yoshinobu, Hamakawa Takashi, Naiki Taku, Ando Ryosuke, Okada Atsushi, Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 24 Issue: 4 Pages: 326-329
  • DOI: 10.1111/iju.13319
  • Peer Reviewed
• [Journal Article] Optimizing RNA Extraction of Renal Papilla Biopsy Tissue in Kidney Stone Formers: A New Methodology for Genomic Study. (2017)
  • Author(s): Taguchi K, Usawachintachit M, Hamamoto S, Unno R, Tzou DT, Sherer BA, Wang Y, Okada A, Stoller ML, Yasui T, Chi T.
  • Journal Title: J Endourol Volume: 31 Issue: 9 Pages: 922-929
  • DOI: 10.1089/end.2017.0320
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Current trends and pitfalls in endoscopic treatment of urolithiasis (2017)
  • Author(s): Inoue Takaaki、Okada Shinsuke、Hamamoto Shuzo、Yoshida Takashi、Matsuda Tadashi
  • Journal Title: Int J Urol Volume: 25 Issue: 2 Pages: 121-133
  • DOI: 10.1111/iju.13491
  • Peer Reviewed
• [Journal Article] Determinants of health-related quality of life for patients after urinary lithotripsy: ureteroscopic vs. shock wave lithotripsy. (2017)
  • Author(s): Hamamoto S, Unno R, Taguchi K, Naiki T, Ando R, Okada A, Inoue T, Okada S, AbdelRazek M, Kohri K, Yasui T
  • Journal Title: Urolithiasis Volume: 印刷中 Issue: 2 Pages: 203-210
  • DOI: 10.1007/s00240-017-0972-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] M1/M2-macrophage phenotypes regulate renal calcium oxalate crystal development (2017)
  • Author(s): 70.Taguchi Kazumi, Okada Atsushi, Hamamoto Shuzo, Unno Rei, Moritoki Yoshinobu, Ando Ryosuke, Mizuno Kentaro, Tozawa Keiichi, Kohri Kenjiro, Yasui Takaihro
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 35167-35167
  • DOI: 10.1038/srep35167
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathophysiology-based treatment of urolithiasis. (2017)
  • Author(s): Yasui T, Okada A, Hamamoto S, Ando R, Taguchi K, Tozawa K, Kohri K.
  • Journal Title: Int J Urol Volume: 24 Issue: 1 Pages: 32-38
  • DOI: 10.1111/iju.13187
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficacy of endoscopic combined intrarenal surgery in the prone split-leg position for staghorn calculi. (2015)
  • Author(s): Hamamoto S, Yasui T, Okada A, Koiwa S, Taguchi K, Itoh Y, Kawai N, Hashimoto Y, Tozawa K, Kohri K.
  • Journal Title: J Endourol Volume: 29 Issue: 1 Pages: 19-24
  • DOI: 10.1089/end.2014.0372
  • Peer Reviewed
• [Journal Article] Proinflammatory and Metabolic Changes Facilitate Renal Crystal Deposition in an Obese Mouse Model of Metabolic Syndrome. (2015)
  • Author(s): Taguchi K, Okada A, Hamamoto S, Iwatsuki S, Naiki T, Ando R, Mizuno K, Tozawa K, Kohri K, Yasui T.
  • Journal Title: J Urol. Volume: 194 Issue: 6 Pages: 1787-96
  • DOI: 10.1016/j.juro.2015.07.083
  • Peer Reviewed / Open Access
• [Journal Article] First case report of staghorn calculi successfully removed by mini-endoscopic combined intrarenal surgery in a 2-year-old boy. (2015)
  • Author(s): Taguchi Kazumi, Hamamoto Shuzo, Okada Atsushi, Mizuno Kentaro, Tozawa Keiichi, Hayashi Yutaro, Kohri Kenjiro, Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 22 Issue: 10 Pages: 978-80
  • DOI: 10.1111/iju.12860
  • Peer Reviewed
• [Journal Article] Influence of pelvicaliceal anatomy on stone clearance after flexible ureteroscopy and holmium laser lithotripsy for large renal stones. (2015)
  • Author(s): Inoue Takaaki, Murota Takashi, Okada Shinsuke, Hamamoto Shuzo, Muguruma Kouei, Kinoshita Hidefumi, Matsuda Tadashi
  • Journal Title: Journal of Endourology Volume: 29 Issue: 9 Pages: 998-1005
  • DOI: 10.1089/end.2015.0071
  • Peer Reviewed
• [Presentation] 尿管所見から考える嵌頓結石に対する理想的な治療介入時期の検討 (2017)
  • Author(s): 濵本 周造、岡田 真介、井上 貴昭、杉野 輝明、海野 奈央子、海野 怜、山田 健司、安藤 亮介、岡田 淳志、三浦 浩康、松田 公志、安井 孝周
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] Successful results of endoscopic combined intrarenal surgery against large calculi; simultaneous use of flexible-ureteroscopy and mini-percutaneous nephrolithotomy. (2017)
  • Author(s): Hamamoto Shuzo, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Fujii Yasuhiro, Ando Ryosuke, Okada Atsushi, Tozawa Keiichi, Yasui Takahiro
  • Organizer: 第105回日本泌尿器科学会総会
  • Int'l Joint Research
• [Presentation] Determinants of health-related quality of life after surgery: ureteroscopic versus shock wave lithotripsy (2017)
  • Author(s): Hamamoto Shuzo, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Shinsuke, Inoue Takaaki, Okada Atsushi, Tozawa Keiichi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] A new navigation system of renal puncture for endoscopic combined intrarenal surgery: real-time virtual sonography-guided renal access. (2017)
  • Author(s): Hamamoto Shuzo, Sugino Teruaki , Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Shinsuke, Inoue Takaaki, Okada Atsushi, Tozawa Keiichi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] Prevalence of urolithiasis in Japan: the results of the nation-wide surveys. (2017)
  • Author(s): Hamamoto Shuzo
  • Organizer: Endourological Society Global Education Initiative
  • Int'l Joint Research
• [Presentation] Investigation of the ideal position for percutaneous nephrolithotomy: prone position vs. modified Valdivia position (2016)
  • Author(s): Hamamoto Shuzo, Unno Rei, Taguchi Kazumi, Okada Shinsuke, Inoue Takaaki, Murota Takashi, Ando Ryosuke, Tozawa Keiichi, Matsuda Tadasi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: 13th International Symposium on Urolithiasis
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2016-07-19
  • Int'l Joint Research
• [Presentation] Genome wide gene expression profiling of Randall plaques in calcium oxalate stone formers (2016)
  • Author(s): Hamamoto Shuzo, Taguchi Kazumi, Sugino Teruaki, Unno Rei, Hirose Masahito, Ando Ryosuke, Okada Atsushi, Tozawa Keiichi, Chi Thomas, Stoller L Marshall, Kohri Kenjiro, Yasui Takahiro
  • Organizer: 13th International Symposium on Urolithiasis
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2016-07-19
  • Int'l Joint Research
• [Presentation] Efficacy of real-time virtual sonography-guided renal access for endoscopic combined intrarenal surgery (2016)
  • Author(s): Hamamoto Shuzo, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Atsushi, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2016
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] PNL-TUL併用療法による残石ゼロを目指した手術治療 (2016)
  • Author(s): 濵本 周造、田口 和己、安藤 亮介、岡田 淳志、戸澤 啓一、郡 健二郎、安井 孝周
  • Organizer: 第104回日本泌尿器科学会総会
  • Place of Presentation: 仙台国際センター他(宮城県仙台市)
  • Year and Date: 2016-04-23
• [Presentation] 結石をあなどるな －嵌頓結石 嵌頓結石に対するTULの実際。 (2015)
  • Author(s): 濵本 周造、岡田 淳志、安井 孝周
  • Organizer: 第29回日本泌尿器内視鏡学会総会
  • Place of Presentation: 京王プラザホテル(東京都新宿区)
  • Year and Date: 2015-11-19
• [Presentation] ＣＴでの微小結石に着目したクエン酸製剤の結石予防効果 (2015)
  • Author(s): 海野 怜、田口 和己、浜本 周造、岡田 淳志、安井 孝周、郡 健二郎
  • Organizer: 日本尿路結石症学会第25回学術集会
  • Place of Presentation: ロワジールホテル旭川(北海道旭川市)
  • Year and Date: 2015-08-28
• [Presentation] Determinants of health-related quality of life for patients after urinary lithotripsy: ureteroscopic versus shock wave lithotripsy. (2015)
  • Author(s): Hamamoto Shuzo, Yasui Takahiro, Okuda Naoko, Koiwa Satoshi, Okada Atsushi, Kamiya Hiroyuki, Hashimoto Yoshihiro, Tozawa Keiichi, Iwase Yutaka, Kohri Kenjiro
  • Organizer: American Urological Association Annual Meeting 2015
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] 経皮的腎砕石術におけるReal-time virtual sonography (RVS)を用いた腎瘻穿刺法の確立。 (2015)
  • Author(s): 濵本 周造、安井 孝周、奥田 奈保子、小岩 哲、内木 拓、岡田 淳志、神谷 浩行、藤田 圭治、橋本 良博、戸澤 啓一、岩瀬 豊、郡 健二郎
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 石川県立音楽堂、ANAクラウンプラザホテル金沢他(石川県金沢市)
  • Year and Date: 2015-04-18