Mechanisms underlying phosphorylation of alpha-crystallin in diabetic retinopathy
| Project/Area Number |
15K10856
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kase Satoru 北海道大学, 大学病院, 講師 (60374394)
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| Co-Investigator(Kenkyū-buntansha) |
神田 敦宏 北海道大学, 医学研究院, 特任講師 (80342707)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | αークリスタリン / VEGF / 糖尿病網膜症 / αBークリスタリン / 病理組織学 / リン酸化 / 血管新生 |
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| Outline of Final Research Achievements |
We found that retinal pigment epithelium (RPE)/choroid thickness was significantly increased from 4 to 8 weeks after streptozotocin (STZ) stimulation, which reached maximum at 8 weeks. Histopathology demonstrated thickened choroid comprising stromal accumulation of some materials and pigments as well as small vessels 8 weeks after stimulation. We further examined protein concentrations using ELISA system. VEGF and alphaB-crystallin concentrations in RPE/choroid proteins were significantly lower in 8 weeks after STZ mice than 4 weeks or control mice. Retinal tissue proteins also revealed similar VEGF/alphaB-crystallin concentrations. We now try to analyze mechanisms underlying such down-regulation of VEGF/alphaB-crystallin in STZ-induced diabetic mice.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Regulation of vascular endothelial growth factor-C by tumor necrosis factor-α in the conjunctiva and pterygium.2016
Author(s)
Dong Y, Kase S, Dong Z, Fukuhara J, Tagawa Y, Ishizuka ET, Murata M, Shinmei Y, Ohguchi T, Kanda A, Noda K, Ishida S.
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Journal Title
Int J Mol Med
Volume: 38
Pages: 545-550
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phosphorylation of alphaB-crystallin in epiretinal membrane of human proliferative diabetic retinopathy.2016
Author(s)
Dong Y, Dong Z, Kase S, Ando R, Fukuhara J, Kinoshita S, Inafuku S, Tagawa Y, Ishizuka ET, Saito W, Murata M, Kanda A, Noda K, Ishida S
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Journal Title
Int J Ophthalmol
Volume: 9
Pages: 1100-1105
DOI
Related Report
Peer Reviewed / Open Access
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