| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To improve the cultivation method, we assumed a limited autologous tissue and performed spheroidal cultivation from human small limbal tissue. Uniformly small and round spheroids were formed even from small limbal tissue. Efficiency of spheroidal formation was differed in the derived site of the original limbus. Spheroids derived from the high spheroidal formation site showed the large colony forming efficiency and higher ratio of slow cycling cells. In addition, N-cadherin was infrequently-expressed in epithelial spheroid. Spheroids were maintained post-operative adaptation and epithelial proliferation in a limbal-deficient rabbit transplantation model.
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