Induction of Muller glia-derived retinal regeneration by expressing regeneration promoting genes

• Project/Area Number: 15K10914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: 独立行政法人国立病院機構(東京医療センター臨床研究センター)
• Principal Investigator: Okamoto Akiko 独立行政法人国立病院機構(東京医療センター臨床研究センター), 分子細胞生物学研究部, 研究員 (70450400)
• Co-Investigator(Renkei-kenkyūsha): IWATA Takeshi 独立行政法人国立病院機構, 東京医療センター臨床研究センター, 部長 (90374157)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 網膜 / グリア細胞 / 再生 / 変性モデル / 傷害 / 細胞増殖 / アデノ随伴ウイルス

Outline of Final Research Achievements

Recovery of visual function is a recent issue for regenerative medicine. Several methods, such as iPS cell-based retinal cell/tissue transplantation, recovery of visual function using channel Rhodopsin and artificial retinal implant, are in progress. On the other hand, retinal Muller glia is focused as an injury-induced retinal stem/progenitor cell in vertebrate species. Regeneration of retinal neuronal cells from Muller glia has also been studied. In this study, we examined regeneration potential of mouse Muller glia by inducing candidate regeneration promoting genes by using adeno-associated virus transfection.

Research Products

• [Journal Article] Identification of novel mutations in the LRR-cap domain of C21orf2 in Japanese patients with retinitis pigmentosa and cone-rod dystrophy. (2016)
  • Author(s): Suga A, Mizota A, Kato M, Kuniyoshi K, Yoshitake K, Sultan W, Yamazaki M, Shimomura Y, Ikeo K, Tsunoda K, Iwata T.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 57 Issue: 10 Pages: 4255-4263
  • DOI: 10.1167/iovs.16-19450
  • Peer Reviewed
• [Presentation] Chracterization and Whole-Genome Analysis of Cynomolgus Monkeys with Hereditary Macular Drusen. (2017)
  • Author(s): Akiko Suga, Mao Nakayama, Zai-Long Chi, Atsushi Mizota, Nobuhiro Shimozawa, Kazutoshi Yoshitake, Takeshi Iwata
  • Organizer: ARVO 2017
  • Int'l Joint Research
• [Presentation] Characterization and whole genome analysis of cynomolgus monkeys with hereditary macular drusen (2016)
  • Author(s): Akiko Suga, Mao Nakayama, Zai-Long Chi, Nobuhiro Shimozawa, Kazutoshi Yoshitake, Takeshi Iwata
  • Organizer: XXII Biennial Meeting of the International Society for Eye Research
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] Identification of novel mutations in C21ORF2 from Japanese patients with early-onset retinitis pigmentosa. (2016)
  • Author(s): Akiko Suga, Mitsuhiro Kato, Kazutoshi Yoshitake, Astushi Mizota, Kazuho Ikeo, Takeshi Iwata
  • Organizer: 13th International Congress of Human Genetics (ICHG2016)
  • Place of Presentation: 京都国際会館
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] 網膜色素変性と錐体杆体ジストロフィーそれぞれの患者から見つかった繊毛遺伝子C21orf2の新規変異 (2016)
  • Author(s): 須賀晶子、溝田淳、加藤光広、国吉一樹、吉武和敏、William Sultan、山崎正志、下村嘉一、池尾一穂、角田和繁、岩田岳
  • Organizer: 第9回 Retina Research Meeting (RRM)
  • Place of Presentation: JPタワー ホール＆カンファレンス
• [Presentation] 全エクソン解析による網膜色素変性の新規原因遺伝子の同定と変異の機能解析 (2015)
  • Author(s): 須賀 晶子
  • Organizer: 国立病院機構東京医療センター 若手研究者発表会2015
  • Place of Presentation: 東京医療センター
  • Year and Date: 2015-11-02
• [Presentation] Proliferation potential of Muller glia after retinal damage varies between mouse strains (2015)
  • Author(s): Akiko Suga
  • Organizer: ARVO 2015
  • Place of Presentation: Colorado convention center (Denver, Colorado)
  • Year and Date: 2015-05-03
  • Int'l Joint Research
• [Remarks] Iwata Laboratory
  • URL: http://www.iwata-lab.jp/