The protective role of heme oxygenase-1 and autophagy in the kidney rhabdomyolysis-associated acute kidney injury
| Project/Area Number |
15K10980
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井上 一由 岡山大学, 医学部, 客員研究員 (10624413)
森松 博史 岡山大学, 医歯薬学総合研究科, 教授 (30379797)
高橋 徹 岡山県立大学, 保健福祉学部, 教授 (40252952)
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| Research Collaborator |
YAMAOKA Masakazu
OMORI Emiko
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 集中治療学 / 急性腎不全 / 横紋筋融解症 / ヘムオキシゲナーゼー１ / Bach1 / オートファジー / ヘムオキシゲナーゼ / ヘムオキシゲナーゼ－１ / Nrf2 / ヘムオキシゲナーゼ-１ |
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| Outline of Final Research Achievements |
We demonstrate that nuclear Bach1 protein was rapidly and significantly decreased in the kidneys of rats with glycerol-associated RM-AKI, followed by an increase in Bach1 protein in the cytosol, which was preceded by the induction of Bach1 mRNA. We detected a significant increase in HO-1 expression and the robust inhibition of ALAS1 expression in the kidneys of glycerol-treated animals, suggesting a significant increase in the free
heme concentration in the kidney of glycerol-treated animals. Bach1 is a heme responsive transcription repressor of the HO-1 gene, and our findings suggest that changes in the subcellular distribution of Bach1 may be involved in the induction of HO-1 accompanying heme metabolism in the kidney of the rat RM-AKI model. To the best of our knowledge, this is the first study to show dynamic changes in renal Bach1 expression in vivo, which were associated with heme metabolism.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Improvement of heme oxygenase-1-based heme sensor for quantifying free heme in biological samples2015
Author(s)
Junichi Taira, Yukinori Nakashima, Shun Yoshihara, Shinya Koga, Shinji Sueda, Hideyuki Komatsu, Yuichiro Higashimoto, Toru Takahashi, Nohito Tanioka, Hiroko Shimizu, Hiroshi Morimatsu, Hiroshi Sakamoto
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Journal Title
Analytical Biochemistry
Volume: 489
Pages: 50-52
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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