Pathogenicity acquisition mechanism of oral streptococci via gene transfer and expression of cytolysin genes
| Project/Area Number |
15K11012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
Nagamune Hideaki 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (40189163)
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| Co-Investigator(Kenkyū-buntansha) |
友安 俊文 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授 (20323404)
田端 厚之 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 講師 (10432767)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 口腔連鎖球菌 / 細胞溶解毒素 / 溶血毒素 |
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| Outline of Final Research Achievements |
In oral streptococci such as mitis group streptococci (MGS) and anginosus group streptococci (AGS), we investigated the search and characterization of unknown molecule of hemolytic cytolysin (HL), the relation between the regulation of HL expression and the pathogenicity, and the mechanism of pathogenicity acquisition via HL gene transfer in S. mitis (SM). In a S. infantis strain belonging to MGS, a HL that is most-closely related to 5 domain-type cholesterol-dependent HL (5D-CDC) of SM and shows quite similar property to 5D-CDC was discovered. This suggests the HL gene transfer among MGS species. Moreover, it was suggested that SM might obtain pathogenicity via HL gene transfer that IS participated in, because the 5D-CDC gene transfer was detected by cultivation of a SM strain with no HL gene in the presence of the genomic DNA from the strains with 5D-CDC gene and IS at the adjacent loci and a competence-stimulating peptide of SM.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Positive- and Negative-Control Pathways by Blood Components for Intermedilysin Production in Streptococcus intermedius2017
Author(s)
Tomoyasu, T., Yamasaki, T., Chiba, S., Kusaka, S., Tabata, A., Whiley, R.A., Nagamune, H.
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Journal Title
Infection and Immunity
Volume: 85(9)
Issue: 9
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene2017
Author(s)
Tomoyasu, T., Matoba, M., Takao, A., Tabata, A., Whiley, R.A., Maeda, N., Nagamune, H.
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Journal Title
FEMS Microbiology Letters
Volume: 365
Issue: 3
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Positive and negative control mechanisms of pathogenicity expression in Streptococcus intermedius2017
Author(s)
Tomoyasu, T., Tabata, A., Chiba, S., Yamasaki, T., Kusaka, S., Takeda, N., Tamaoka, M., Ohkura, K., Whiley, R.A., Nagamune , H.
Organizer
20th Lancefield International Symposium on Streptococci and Streptococcal Diseases
Related Report
Int'l Joint Research
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[Presentation] Characterization of a novel plasmid discovered in a clinical isolate of Streptococcus anginosus subsp. anginosus2017
Author(s)
Tabata, A., Deutsch, D., Otsuka, S., Verratti, K., Tomoyasu, T., Nagamune, H., Fischetti, V.A.
Organizer
20th Lancefield International Symposium on Streptococci and Streptococcal Diseases
Related Report
Int'l Joint Research
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