Analysis of mechanism for pro-inflammatory and anti-inflammatory balance and role of Th17 cells in pulpitis

• Project/Area Number: 15K11115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NAKANISHI Tadashi 徳島大学, 大学院医歯薬学研究部(歯学系), 准教授 (00217770)
• Co-Investigator(Kenkyū-buntansha): 湯本 浩通 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (60284303) ; 細川 義隆 徳島大学, 病院, 講師 (90346601)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯髄炎 / 象牙芽細胞 / Th17 / 歯髄 / 炎症 / サイトカイン / IL-17 / ケモカイン

Outline of Final Research Achievements

The purpose of this study was to elucidate the role of Th17 cells in the pathogenesis of pulpitis, and thus we examined the effect of interleukin(IL)-17 on CCL20 production from KN-3 cells established as rat odontoblast-like cells.
IL-17 increased CCL-20 production from KN-3 cells exposed to IL-1. The treatment of IL-17 also enhanced the phosphorylation of mitogen-activated protein kinases (MAPKs) in IL-1 stimulated KN-3 cells. Moreover, epigallocatechin-3 gallate, a representative type of catechins, reduced CCL20 production from activated KN-3 cells.

Research Products

• [Journal Article] Anti-inflammatory Effects of Catechin on Rat Odontoblastic Cells (KN-3) (2017)
  • Author(s): 平尾功治、湯本浩通、細川由樹、蔵本 瞳、鷲尾絢子、中西 正、武川大輔、北村知昭、松尾敬志
  • Journal Title: The Japanese Journal of Conservative Dentistry Volume: 60 Issue: 5 Pages: 235-244
  • DOI: 10.11471/shikahozon.60.235
  • NAID: 130006191168
  • ISSN: 0387-2343, 2188-0808
  • Peer Reviewed / Open Access
• [Presentation] Effect of interleukin-17A on CCL20 production from odontoblast-like cells. (2017)
  • Author(s): Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Takashi Matsuo
  • Organizer: 95th General Session & Exhibition of the IADR
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Interleukin-17がラット象牙芽細胞様細胞（KN-3）のCCL20産生に及ぼす影響 (2015)
  • Author(s): 中西 正、武川大輔、平尾功治、湯本浩通、細川由樹、松尾敬志
  • Organizer: 日本歯科保存学会 2015年度秋季学術大会（第143回）
  • Place of Presentation: 文京シビックホール（東京都・文京区）
  • Year and Date: 2015-11-12