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Epigenetic regulation of cellular robustness in DNA damage response

Research Project

Project/Area Number 15K12203
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionKyoto University

Principal Investigator

Ikura Tsuyoshi  京都大学, 放射線生物研究センター, 准教授 (70335686)

Co-Investigator(Renkei-kenkyūsha) MATSUDA Tomonari  京都大学, 工学研究科附属 流域圏総合環境質研究センター, 准教授 (50273488)
Research Collaborator IKURA Masae  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords放射線障害 / エピジェネティクス / TIP60 / アセチル化 / 細胞ロバストネス
Outline of Final Research Achievements

We investigated the mechanism of acquired radio-resistance in cells after gamma irradiation, focusing on histone modifications and protein networks via histone modifications around DNA damage sites. In order to clarify this, we performed chromatin immunoprecipitation (Chromatin IP) after infection I-Sce1 adenovirus, which produce DNA double-strand break, in the acquired radio-resistance HeLa/DR-GFP cells, using anti-histone H2AX acetylation, phosphorylation or histone H4 acetylation antibodies. As a result, no remarkable change of histone modifications was observed between the acquired radio-resistance and non-resistant HeLa/DR-GFP cells. We also analyzed DNA repair efficiency using HeLa/DR-GFP system between these cells. As a result, DNA repair efficiency in acquired radio-resistance HeLa/DR-GFP cells is comparable to that in non-resistant HeLa/DR-GFP cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (36 results)

All 2017 2016 2015

All Journal Article (14 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 14 results,  Open Access: 10 results,  Acknowledgement Compliant: 2 results) Presentation (22 results) (of which Int'l Joint Research: 2 results,  Invited: 8 results)

  • [Journal Article] Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3.2017

    • Author(s)
      Kakumu E
    • Journal Title

      Genes Cells.

      Volume: 3 Issue: 3 Pages: 310-327

    • DOI

      10.1111/gtc.12479

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells.2016

    • Author(s)
      Kaimori, J. Y., Maehara, K., Hayashi-Takanaka, Y., Harada, A., Fukuda, M., Yamamoto, S., Ichimaru, N., Umehara, T., Yokoyama, S., Matsuda, R., Ikura, T., Nagao, K., Obuse, C., Nozaki, N., Takahara, S., Takao, T., Ohkawa, Y., Kimura, H., and Isaka, Y.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 24318-24318

    • DOI

      10.1038/srep24318

    • NAID

      120005770760

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Histone H2A T120 Phosphorylation Promotes Oncogenic Transformation via Upregulation of Cyclin D1.2016

    • Author(s)
      Aihara H
    • Journal Title

      Mol. Cell.

      Volume: 64 Issue: 1 Pages: 176-188

    • DOI

      10.1016/j.molcel.2016.09.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coordinated regulation of TIP60 and PARP-1 in damaged chromatin dynamics.2016

    • Author(s)
      Ikura M
    • Journal Title

      Mol. Cell. Biol.

      Volume: Epub ahead of print Issue: 10 Pages: 1595-1607

    • DOI

      10.1128/mcb.01085-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disruption of DNA Damage-Response by Propyl Gallate and 9-Aminoacridine2016

    • Author(s)
      Matsuda S
    • Journal Title

      Toxicol. Sci.

      Volume: 150 Issue: 2 Pages: 1-12

    • DOI

      10.1093/toxsci/kfw039

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] SMARCAD1 is an ATP-dependent stimulator of nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation.2016

    • Author(s)
      Doiguchi M
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 20179-20179

    • DOI

      10.1038/srep20179

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3*2016

    • Author(s)
      Tanaka H
    • Journal Title

      JBC

      Volume: 291 Issue: 12 Pages: 6316-6330

    • DOI

      10.1074/jbc.m116.713842

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Acetylation of Histone H2AX at Lys 5 by the TIP60 Histone Acetyltransferase Complex Is Essential for the Dynamic Binding of NBS1 to Damaged Chromatin.2015

    • Author(s)
      Ikura M
    • Journal Title

      Mol. cell. Biol.

      Volume: 35 Issue: 24 Pages: 4147-4157

    • DOI

      10.1128/mcb.00757-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear pyruvate kinase M2 complex serves as a transcriptional coactivator of arylhydrocarbon receptor.2015

    • Author(s)
      Shun Matsuda, Jun Adachi, Masaru Ihara, Nobuhiro Tanuma, Hiroshi Shima, Akira Kakizuka, Masae Ikura, Tsuyoshi Ikura, Tomonari Matsuda
    • Journal Title

      Nucleic Acids Res

      Volume: 44(2) Issue: 2 Pages: 636-647

    • DOI

      10.1093/nar/gkv967

    • NAID

      120005818255

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin2015

    • Author(s)
      Arimura Y
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1 Pages: 09628-09628

    • DOI

      10.1038/srep09628

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] hCAS/CSE1L regulates RAD51 distribution and focus formation for homologous recombinational repair2015

    • Author(s)
      1.Okimoto S, Sun J, Fukuto A, Horikoshi Y, Matsuda S, Matsuda T, Ikura M, Ikura T, Machida S, Kurumizaka H, Miyamoto Y, Oka M, Yoneda Y, Kiuchi Y, Tashiro S.
    • Journal Title

      Genes Cells.

      Volume: 20 Issue: 9 Pages: 681-94

    • DOI

      10.1111/gtc.12262

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Absolute quantification of acetylation and phosphorylation of the histone variant H2AX upon ionizing radiation reveals distinct cellular responses in two cancer cell lines2015

    • Author(s)
      Matsuda S
    • Journal Title

      Radiat. Environ. Biophys.

      Volume: 54 Issue: 4 Pages: 403-411

    • DOI

      10.1007/s00411-015-0608-3

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] SUMOylation of xeroderma pigmentosum group C protein regulates DNA damage recognition during nucleotide excision repair2015

    • Author(s)
      Akita M
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1 Pages: 10984-10984

    • DOI

      10.1038/srep10984

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Absolute quantification of γH2AX using liquid chromatography-triple quadrupole tandem mass spectrometry2015

    • Author(s)
      Matsuda, S., Ikura, T., Matsuda, T.
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: in press Issue: 18 Pages: 5521-5527

    • DOI

      10.1007/s00216-015-8725-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] タンパク質複合体解析の現状と今後への挑戦2016

    • Author(s)
      井倉 毅
    • Organizer
      岡崎フラグメント50周年シンポジウム
    • Place of Presentation
      名古屋市
    • Year and Date
      2016-12-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゲノムストレス応答の多様性を数理的アプローチで理解する2016

    • Author(s)
      井倉 毅
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] リンカーヒストンH1を含むクロマチンでの相同組換え反応2016

    • Author(s)
      町田晋一
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] RAD51による相同組換え修復の制御機構2016

    • Author(s)
      堀越保則
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] DNAチェックポイント因子Rad9の機能制御を担うCdk-Plk1依存的機構の意義2016

    • Author(s)
      郡司 未佳
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] がん細胞で高頻度におこるヒストンH2B点変異体はヌクレオソーム構造および細胞増殖に影響を与える2016

    • Author(s)
      野田真美子
    • Organizer
      第39回 日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular mechanism of etoposide induced 11q23 chromosome translocations.2016

    • Author(s)
      孫 継英
    • Organizer
      日本放射線影響学会 第59回大会
    • Place of Presentation
      広島市
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] がん研究入門コース1「がん研究におけるクロマチン生物学」2016

    • Author(s)
      井倉 毅
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] DNA損傷応答におけるヒストンH2AX複合体モジュールのダイナミクス2016

    • Author(s)
      井倉 毅
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular mechanism of etoposide induced 11q23 chromosome translocations.2016

    • Author(s)
      Jiying Sun
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] クロマチンの動的変化に着目したゲノム損傷応答の多様性の理解2016

    • Author(s)
      井倉 毅
    • Organizer
      日本環境変異原学会・変異機構研究会・第29回 夏の学校
    • Place of Presentation
      京都府京北町
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] クロマチン制御から見たDNA 損傷応答研究の現状と今後の展望2016

    • Author(s)
      井倉 毅
    • Organizer
      東北大学加齢医学研究所セミナー
    • Place of Presentation
      仙台市
    • Year and Date
      2016-01-15
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] ヒストン化学修飾から見たDNA修復研究の展望2015

    • Author(s)
      井倉 毅
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学学会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] DNA損傷応答における動的クロマチン平衡とその意義2015

    • Author(s)
      井倉 毅
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] DNAチェックポイント因子Rad9の分解を促進するCdk-Plk1依存的機構2015

    • Author(s)
      郡司未佳
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] ピルビン酸キナーゼとピルビン酸デヒドロゲナーゼ複合体によるアセチルCoAの治産池消モデル2015

    • Author(s)
      松田俊
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Involvement of a chromatin remodeling factor in chromosomal translocations2015

    • Author(s)
      孫継英
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] アセチル化を介した損傷クロマチンダイナミクス2015

    • Author(s)
      井倉 毅
    • Organizer
      横浜市立大学生命医科学研究科セミナー
    • Place of Presentation
      横浜市
    • Year and Date
      2015-11-17
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Molecular mechanism of etoposide induced 11q23 chromosome translocation. Etoposideによる11q23 染色体転座形機構の解明2015

    • Author(s)
      Jiying Sun
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] The Role of Histone H2AX Dynamics in DNA Damage Response2015

    • Author(s)
      Tsuyoshi Ikura
    • Organizer
      第15回国際放射線研究会議(ICRR2015)
    • Place of Presentation
      京都市
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Acetylation-dependent positive feedback loop regulation in dynamic chromatin equilibrium2015

    • Author(s)
      Tsuyoshi Ikura
    • Organizer
      大阪大学蛋白研セミナー、Institute for Protein Research (IPR) Seminar
    • Place of Presentation
      吹田市
    • Year and Date
      2015-05-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Chromatin dynamics in DNA damage response2015

    • Author(s)
      Tsuyoshi Ikura
    • Organizer
      First DSV/CEA-RBC joint workshop
    • Place of Presentation
      France
    • Year and Date
      2015-04-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2018-03-22  

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