Research Project
Grant-in-Aid for Challenging Exploratory Research
Peptidic natural products often consist of not only simple amide bonds but also modified backbones. Since such unique backbones are important structural motifs that contribute to diverse bioactivities, peptides with modified backbones can also be attractive candidates for artificial bioactive peptides. This research program devised an in vitro biosynthesis system referred to as FIT-PatD system by the integration of an engineered cell-free translation system with a posttranslational cyclodehydratase, enabling “one-pot” synthesis of a wide variety of azoline-containing peptide derivatives expressed from synthetic DNA templates. Moreover, we have demonstrated in vitro biosynthesis of peptides with various backbone structures by combination of the FIT-PatD system and chemical modifications. These results offer the potentials for the production of a wide collection of backbone-modified peptides and may facilitate the discovery of “peudo-natural” bioactive peptides.
All 2018 2017 2016 2015
All Journal Article (10 results) (of which Int'l Joint Research: 6 results, Peer Reviewed: 9 results, Open Access: 6 results, Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 5 results, Invited: 13 results)
YAKUGAKU ZASSHI
Volume: 138 Issue: 1 Pages: 55-61
10.1248/yakushi.17-00186-3
130006300866
RNA Biol.
Volume: 15 Issue: 4-5 Pages: 576-585
10.1080/15476286.2017.1353846
Methods Mol Biol.
Volume: 1728 Pages: 17-47
10.1007/978-1-4939-7574-7_2
Nat Commun
Volume: 8 Issue: 1 Pages: 14773-14773
10.1038/ncomms14773
Proc Natl Acad Sci U S A
Volume: 114 Issue: 33
10.1073/pnas.1703925114
Mol Pharm
Volume: 14 Issue: 10 Pages: 3290-3298
10.1021/acs.molpharmaceut.7b00362
J Am Chem Soc
Volume: 138 Issue: 6 Pages: 1962-1969
10.1021/jacs.5b12482
J Mol Evol
Volume: 81 Issue: 5-6 Pages: 210-217
10.1007/s00239-015-9710-z
Volume: 112 Issue: 19 Pages: 6027-6032
10.1073/pnas.1423664112
Peptide Science
Volume: 52 Pages: 27-28
