Thermodynamics for Single/Multicellular Transition

• Project/Area Number: 15K13617
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical chemistry
• Research Institution: Saitama University
• Principal Investigator: NAKABAYASHI Seiichiro 埼玉大学, 理工学研究科, 教授 (70180346)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 単細胞多細胞転移 / 細胞性粘菌 / NADH蛍光 / ATP / NADH / 顕微蛍光測定 / 代謝 / C-dot / fluorescent probes / bioimaging

Outline of Final Research Achievements

A plausible reason of an evolution of a multicellular organism from single cellular one was studied experimentally by using starvation induced aggregation of Dictyostelium. While the total number of Dictyostelium cells were constant, WST measurement showed decrease in total NADH by the aggregation. The amount of NADH in a single cell was detected by fluorescence microscopy. A collision of two free moving cells decreased the fluorescence intensity. After separation, the intensity was recovered. These two types of measurements were harmonized well to indicate that the multicellular organism requested less energy dissipation than that of single cellular system, which means the biological transition of single to multicellular organism was achieved by the thermodynamically.

Research Products

• [Int'l Joint Research] Hungary Academy of Sciences(Hungary)
• [Int'l Joint Research] Indian Institute of Technology Bombay(India)
• [Journal Article] Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins (2016)
  • Author(s): Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii
  • Journal Title: In Vitro Cellular & Developmental Biology -Animal Volume: 52 Issue: 8 Pages: 799-805
  • DOI: 10.1007/s11626-016-0049-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Kinesin-driven active substrate giving stochastic mechanical stimuli to cells for characterization (2016)
  • Author(s): Ryuzo Kawamura, Daiki Uehara, Naritaka Kobayashi, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa
  • Journal Title: ACS Biomaterials Science & Engineering Volume: 2 Issue: 12 Pages: 2333-2338
  • DOI: 10.1021/acsbiomaterials.6b00538
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photosensitizer-Conjugated Ultrasmall Carbon Nanodots as Multifunctional Fluorescent Probes for Bioimaging (2016)
  • Author(s): A.Loukanov, R.Sekiya, M.Yoshikawa, N.Kobayashi,Y. Moriyasu, S. Nakabayashi
  • Journal Title: J.Phys.Chem.C Volume: 120 Issue: 29 Pages: 15867-15874
  • DOI: 10.1021/acs.jpcc.5b11721
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Investigation on Nanoscale Processes on the BaF2(111) Surface in Various Solutions by Frequency Modulation Atomic Force Microscopy (2016)
  • Author(s): N,Kobayashi, R.Kawamura, H.Yoshikawa, S.Nakabayashi.
  • Journal Title: J.Appl.Phys. Volume: 119 Issue: 21 Pages: 214308-214308
  • DOI: 10.1063/1.4953233
  • Peer Reviewed
• [Journal Article] Synchronization using environmental coupling in mercury beating heart oscillators (2016)
  • Author(s): T. Singla, F.Montoya, M.Rivera, S.Tajima, S.Nakabayashi, P.Parmananda.
  • Journal Title: Chaos Volume: 26 Issue: 6 Pages: 063103-063103
  • DOI: 10.1063/1.4953014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy (2016)
  • Author(s): 4)T. Matsuzaki, K. Ito, K. Masuda, E. Kakinuma, R. Sakamoto, K. Iketaki, H. Yamamoto, M. Suganuma, N. Kobayashi, S. Nakabayashi, T. Tanii, H. Y. Yoshikawa
  • Journal Title: Journal of Physical Chemistry B Volume: 120 Issue: 7 Pages: 1221-1227
  • DOI: 10.1021/acs.jpcb.5b11870
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A temporal semi-stochastic model for pitting corrosion (2016)
  • Author(s): H.Singh, S.Nakabayashi.
  • Journal Title: J.Electroanal.Chem. Volume: 766 Pages: 60-70
  • DOI: 10.1016/j.jelechem.2016.01.033
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Intracellular Imaging of Qdots-Labeled DNA in Cyanobacteria (2016)
  • Author(s): A. Loukanov, V. Zhelyazkov,Y. Hihara, Yukako, S. Nakabayashi,
  • Journal Title: MICROSCOPY RESEARCH AND TECHNIQUE. Volume: 79 Issue: 5 Pages: 447-452
  • DOI: 10.1002/jemt.22651
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The emergence of synchrony behavior in weakly coupled electrochemical oscillators via a 'metallic plate (2016)
  • Author(s): S.Tajima, H.Singh, S. Nakabayashi, T. Singla, P. Parmananda
  • Journal Title: J.Electroanal.Chem. Volume: 769 Pages: 16-20
  • DOI: 10.1016/j.jelechem.2016.03.004
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Photosensitizer-Conjugated Ultrasmall Carbon Nanodots as Multifunctional Fluorescent Probes for Bioimaging (2016)
  • Author(s): Seiichiro Nakabayashi
  • Organizer: Advanced Architectures in Photonics 2016
  • Place of Presentation: Mykonos Island, Greece
  • Year and Date: 2016-09-25
  • Int'l Joint Research / Invited
• [Remarks] Nakabayashi-Yoshikawa Lab
  • URL: http://park.saitama-u.ac.jp/~nakabayashi-lab/index.php?e/FrontPage
• [Funded Workshop] Advanced Architectures in Photonics 2016 (2016)
  • Place of Presentation: Mykonos Island, Greece
  • Year and Date: 2016-09-25