Development of novel method for generation of genome editing mice without ex vivo handling of embryos
| Project/Area Number |
15K14371
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
和田 健太 東京農業大学, 生物産業学部, 准教授 (20508113)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Masahiro 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 教授 (30287099)
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| Research Collaborator |
MIURA Hiromi 東海大学, 医学部, 特定研究員 (90599523)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ゲノム編集 / マウス / CRISPR / エレクトロポレーション / in vivo / GONAD / i-GONAD / CRISPR/Cas9 / 白内障 / piggyBac |
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| Outline of Final Research Achievements |
Animal transgenesis involves three major technical steps; isolation of fertilized eggs, microinjection and subsequent transfer of eggs into recipient females, which require sophisticated equipment as well as highly skilled personnel to perform the task. Genome-editing via Oviductal Nucleic Acids Delivery (GONAD) method developed in this study is a novel technique enabling genome editing in mice through intraoviductal instillation of genome editing components followed by an in vivo electroporation. Since the GONAD does not require ex vivo manipulation of zygotes, it is possible to produce genome-edited mice even by the researchers who do not possess special skills for ex vivo handling and micromanipulation of embryos.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.2016
Author(s)
Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
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Journal Title
Int J Mol Sci.
Volume: 17
Issue: 9
Pages: 1424-1424
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] ex vivo胚操作が不要なゲノム編集マウス作製法2016
Author(s)
大塚 正人, 高橋 剛, 和田 健太, 三浦 浩美, 佐藤 正宏
Organizer
平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
Place of Presentation
蓼科グランドホテル滝の湯（長野県茅野市）
Year and Date
2016-09-14
Related Report
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