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Analysis of Cold-Adaptation Mechanism of Food Spoilage Bacteria and Its Application to Food Industry

Research Project

Project/Area Number 15K14732
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Co-Investigator(Renkei-kenkyūsha) KAWAMOTO Jun  京都大学, 化学研究所, 助教 (90511238)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords低温増殖性細菌 / 食品変敗 / 低温適応機構 / 表層タンパク質 / タンパク質生産 / 膜タンパク質
Outline of Final Research Achievements

We found that Leuconostoc mesenteroides NH04 isolated from spoiled processed meat products has higher adhesion to mucin than the ATCC8292 strain and the NBRC3832 strain of the same species, and its adhesiveness is increased in the presence of sodium nitrite used as a food additive. A homolog of bifunctional acetaldehyde-CoA/alcohol dehydrogenase was identified as a cell-surface protein of NH04 possibly involved in its adhesion. We also identified homologs of proteins involved in RNA modification/folding and transport of compatible solutes as cold-inducible proteins of NH04 that may be involved in its cold adaptation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Proteomic Insights of Psychrophiles2017

    • Author(s)
      Kawamoto, Jun、Kurihara Tatsuo、Esaki Nobuyoshi
    • Journal Title

      Psychrophiles: From Biodiversity to Biotechnology Second Edition

      Volume: - Pages: 423-435

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid2017

    • Author(s)
      Yokoyama F, Kawamoto J, Imai T, Kurihara T
    • Journal Title

      Extremophiles

      Volume: 印刷中 Issue: 4 Pages: 723-731

    • DOI

      10.1007/s00792-017-0937-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a versatile method for targeted gene deletion and insertion by using the pyrF gene in the psychrotrophic bacterium, Shewanella livingstonensis Ac102016

    • Author(s)
      Ito T, Gong C, Kawamoto J, Kurihara T
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 122 Issue: 6 Pages: 645-651

    • DOI

      10.1016/j.jbiosc.2016.06.004

    • NAID

      40021044862

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 低温菌の低温環境への適応メカニズム2015

    • Author(s)
      川本純、栗原達夫
    • Journal Title

      生物工学

      Volume: 93 Pages: 477-480

    • Related Report
      2015 Research-status Report
  • [Presentation] 低温菌 Shewanella sp. HM13 の膜小胞輸送による選択的タンパク質分泌に関与する遺伝子の探索2018

    • Author(s)
      釜阪紘平、陳晨、横山文秋、川本純、今井友也、小川拓哉、栗原達夫
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 低温菌 Shewanella sp. HM13 におけるベシクル分泌関連タンパク質の探索2018

    • Author(s)
      横山文秋、川本純、陳晨、今井友也、栗原達夫
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of a protein secretion mechanism via the membrane vesicle production of a cold-adapted bacterium, Shewanella sp. HM132017

    • Author(s)
      Chen C, Kawai S, Kawamoto J, Imai T, Kurihara T
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 低温菌が分泌する膜小胞の特性解析~膜小胞を基盤とした低温下における異種タンパク質発現系への応用可能性~2017

    • Author(s)
      横山文秋、川本純、今井友也、栗原達夫
    • Organizer
      第64回 生化学会近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アジ腸管由来低温菌のベシクル形成関連タンパク質の同定2017

    • Author(s)
      横山文秋、川本純、今井友也、栗原達夫
    • Organizer
      特殊環境微生物セミナー2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 低温菌 Shewanella sp. HM13 の菌体外膜小胞を介した選択的タンパク質分泌機構の解析2017

    • Author(s)
      釜阪紘平、陳晨、横山文秋、今井友也、川本純、小川拓哉、栗原達夫
    • Organizer
      極限環境生物学会2017年度年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 膜小胞生産性低温適応細菌 Shewanella sp. HM13 によるタンパク質分泌機構の解析2017

    • Author(s)
      陳晨、河合総一郎、川本純、今井友也、栗原達夫
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 低温菌 Shewanella sp. HM13 における菌体外小胞輸送を介したタンパク質輸送に関与する遺伝子の探索2017

    • Author(s)
      釜阪紘平、陳晨、横山文秋、今井友也、川本純、小川拓哉、栗原達夫
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Characterization and application of a psychrotrophic lactic acid bacterium, Leuconostoc mesenteroides NH04, isolated from spoiled meat products2017

    • Author(s)
      Yagura Kazuki、Goto Seitaro、Ninomiya Takemasa、Kawamoto Jun、Ogawa Takuya、Kurihara Tatsuo
    • Organizer
      International Workshop on Microbes in Hostile Environments
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 による菌体外膜小胞の生産とエイコサペンタエン酸生産能の欠損が膜小胞分泌におよぼす影響2016

    • Author(s)
      横山文秋、川本純、今井友也、小川拓哉、栗原達夫
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of protein secretion system of a membrane-vesicle producing cold-adapted bacterium, Shewanella sp. HM132016

    • Author(s)
      Chen C, Kawai S, Kawamoto J, Imai T, Kurihara T
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of eicosapentaenoic acid-containing membrane vesicles produced by a psychrotrophic bacterium, Shewanella livingstonensis Ac102016

    • Author(s)
      Yokoyama F, Kawamoto J, Imai T, Ogawa T, Kurihara T
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の菌体外膜小胞の特性とエイコサペンタエン酸が小胞形成に与える影響の解析2016

    • Author(s)
      横山文秋、川本純、今井友也、小川拓哉、栗原達夫
    • Organizer
      第63回 日本生化学会 近畿支部例会
    • Place of Presentation
      神戸薬科大学(神戸市)
    • Year and Date
      2016-05-21
    • Related Report
      2016 Research-status Report
  • [Presentation] Characterization of a new cold-adapted Shewanella sp. to develop a system for secretory production of proteins2016

    • Author(s)
      陳晨、河合総一郎、川本純、今井友也、栗原達夫
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道 札幌市)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 低温適応性乳酸菌を宿主とした新規ベクターの構築2016

    • Author(s)
      二宮健匡、後藤清太郎、川本純、工藤和幸、栗原達夫
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道 札幌市)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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