Development of artificial enzymes bearing lignin-binding peptide sequence and their delivery system
Project/Area Number |
15K14773
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Wood science
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | リグニン / ペプチド / ラッカーゼ / バイオリファイナリー / バイオマス / リグニン分解酵素 / 白色腐朽菌 / 木材腐朽菌 |
Outline of Final Research Achievements |
The binding behavior of a 12-mer peptide and its tandem dimer to lignin was analyzed. The tandem dimerization increased affinity to lignin by around 10 times. The tandem dimer significantly changed its conformation upon addition of the lignin to have a 10-times higher affinity than the 12-mer peptide. Laccase bearing the tandem dimer peptide was expressed, and reacted to woody biomass pretreated by microwave hot water treatment. It was found that the incorporation of lignin-binding peptide increased the reactivity to lignin and gave higher amount of low molecular mass fragments.
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Report
(3 results)
Research Products
(10 results)
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[Presentation] Analysis of molecular interaction of peptides with lignin for lignocellulosic biorefinery2016
Author(s)
T. Watanabe, A. Yamaguchi, S. Oshiro, T. Suetomi, H. Nishimura, T. Nagata, T. Mashima, M. Katahira, K. Isozaki, H. Takaya, M. Nakamura
Organizer
LignoBiotech IV - 4th Symposium on Biotechnology applied to Lignocelluloses
Place of Presentation
マドリッド
Related Report
Int'l Joint Research
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