Analysis of alternative splicing regulated by CHERP
| Project/Area Number |
15K14919
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Masuda Seiji 京都大学, 生命科学研究科, 准教授 (20260614)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 選択的mRNAスプライシング / CHERP / カルシウム / 遺伝子発現 / 選択的スプライシング |
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| Outline of Final Research Achievements |
Alternative mRNA splicing is a fundamental mechanism to produce plenty of protein more than those encoded in its genome. The understanding of alternative splicing will make us to develop the therapy strategy for diseases based on the inadequate miss-splicing.
CHERP is first identified as Ca2+ signaling related protein in the endoplasmic reticulum. Then, CHERP is also localized in the nucleus, suggesting CHERP has another function. Here, I examined the CHERP regulating genes and alternative splicing. To analyze the genome wide expression change of each mRNA and exon, the exon array analysis was performed. To know the function of CHERP, then, GO term analysis was also carried out. The depletion of CHERP caused the decreased expression of cell cycle, mitosis, cytokinesis, meiosis, DNA repair and DNA replication GO term and increased the induction of apoptosis GO term, suggesting that CHERP has a role for cell proliferation and survival by regulating mRNA splicing.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] The isoflavone fraction from soybean presents mRNA maturation inhibition activity2017
Author(s)
Kurata M, Murata Y, Momma K, Fouad Ali Mursi I, Takahashi M, Miyamae Y, Kambe T, Nagao M, Narita H, Shibuya Y, Masuda S
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Journal Title
Biosci Biotechnol Biochem
Volume: 81
Issue: 3
Pages: 551-554
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The PP-motif in luminal loop 2 of ZnT transporters plays a pivotal role in TNAP activation2016
Author(s)
Fujimoto S, Tsuji T, Fujiwara T, Takeda T, Merriman C, Fukunaka A, Nishito Y, Fu D, Hoch E, Sekler I, Fukue K, Miyamae Y, Masuda S, Nagao M, Kambe T
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Journal Title
Biochem. J
Volume: 473
Issue: 17
Pages: 2611-2621
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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