| Project/Area Number |
15K15080
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山内 祥生 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (00444878)
橋本 登 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (90712365)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | シグレック / 自然免疫 / シアル酸 / チェックポイント / 癌免疫 / 糖鎖 / 細胞傷害 / 糖脂質 / シグナル / 単球 / マクロファージ / アストロサイトーマ |
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| Outline of Final Research Achievements |
Sialic acid-containing cancer-associated carbohydrates are involved in the malignant properties of tumor cells, while innate immune cells such as NK and monocyte-macrophages express sialic-acid-recognizing lectins such as siglec-7 and 9. Then, significance and application for therapeutics of their interaction have been analyzed.
Analyses of carbohydrate structures recognized by siglecs and of their carrier molecules were performed, showing that siglec-7 recognized disialyl glycolipids such as GD3, and tri-sialylated carbohydrate-conjugated proteins. It was also shown that signals mediated by siglec-7 suppressed NK activity. Furthermore, it was demonstrated that GD3 with hydroxylated long chain base at C4 was not recognized by siglec-7, and also glycolipids containing hydroxylated ceramides scarcely localized in lipid rafts.
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